诺氟沙星在鲫体内的药动学及残留研究  

The Pharmacokinetics and Residues of Norfloxacin in Carassius auratus

作　　者：郭海燕[1,2] 马跃岗 陈元坤 张其中[1,3] 毛秀立[1] 孙力华[1] 

机构地区：[1]水产科学重庆市市级重点实验室,西南大学生命科学学院,重庆400715 [2]重庆市水产科学研究所,重庆400020 [3]暨南大学水生生物研究所,广州510632

出　　处：《淡水渔业》2008年第2期46-50,共5页Freshwater Fisheries

基　　金：重庆市科技计划项目(2004-8449)

摘　　要：研究了单剂量肌肉注射和多剂量混饲口灌给药方式下,诺氟沙星在鲫(Carassius auratus)体内的药物动力学和残留情况。结果显示:在(25.4±0.3)℃水温条件下,以每千克鱼体重10 mg的剂量单次给鲫肌肉注射诺氟沙星后,药物几乎在瞬间吸收,0.0333 h时血液中达到6.6708μg/mL,其血药浓度-时间数据用一级吸收二室模型描述较为合适,主要的药动学参数为:t1/2α、t1/2β、AUC和C l(s)分别为:0.4231 h、9.1613 h、17.8619μg.h/mL和0.5727 L/(kg.h)。在(23±1)℃水温条件下,以每千克鱼体重10 mg的剂量多次连续5 d混饲后,鲫停药后第8天肌肉、血清和肝脏中未检测到药物,此时肾脏中药物浓度已降到(0.0463±0.0134)μg/g,低于0.05μg/g。建议在(23±1)℃水温条件下,按每千克体重10 mg的剂量连续5 d混饲给鲫诺氟沙星,休药期至少为最后一次给药后的8 d。The present study investigated pharmacokinetics and residues of norfloxacin （NFX） in Carassius auratus. The drug concentrations in serum, muscle,liver and kidney were determined by Re-high performance liquid chromatography（ Re- HPLC）. The results showed that after a single-dosing intramuscular injection of 10 mg NFX/kg body weight in C. auratus at the water temperature of（25.4 ± 0. 3 ） ℃, the drug concentration in the serum amounted to the largest（6. 6708 μg/mL） immediately at the first sampling point at 0. 033 h, the drug concentration-data in the serum can be described as a two-compartmental open model, the main phannacokinetics parameters are t1/2α, t1/2β, AUC and CI（s）, which are 0. 4231 h, 9. 1613 h, 17. 8619 μg · h/mL and 0. 5727 L/（kg · h） respectively. And no drugs were detected in muscle, serum and liver in C. auratus, the concentration in kidney is（0. 0463 ±0. 0134） μg/g and lower than 0.05 μg/g on Day 8. Therefore, we suggest a withdrawal period should last at least for 8 days in C. auratus at the water temperature of （23 ± 1 ） ℃.

关 键 词：鲫(Carassius auratus) 诺氟沙星 高效液相色谱法 药物动力学 残留 

分 类 号：S948[农业科学—水产养殖]