1,25-(OH)_2D_3对糖尿病肾病大鼠血管内皮生长因子表达的影响  

Effect of 1,25-(OH)_2D_3 on Vascular Endothelial Growth Factor Expression in Rats with Diabetic Nephropathy

作　　者：邹敏书[1] 余健[1] 赵林双[2] 聂国明 刘雪梅[1] 

机构地区：[1]广州军区武汉总医院儿科,武汉430070 [2]广州军区武汉总医院内分泌科,武汉430070

出　　处：《华南国防医学杂志》2008年第1期18-20,31,共4页Military Medical Journal of South China

基　　金：湖北省自然科学基金项目(2002AB116)

摘　　要：目的探讨1,25-(OH)2D3对糖尿病肾病(DN)大鼠肾组织VEGFmRNA及蛋白质表达的影响。方法SD大鼠分为3组,对照组,糖尿病肾病(DN)组,1,25-(OH)2D3组。链脲菌素腹腔注射诱导DM大鼠模型,尿蛋白定量≥30mg/24h为DN大鼠,诱导成功后1,25-(OH)2D3组给予3ng/(100g.d)皮下注射。实验12周末检测大鼠体重、血糖、血肌酐、24h尿白蛋白。采用逆转录聚合酶链反应(RT-PCR)检测VEGFmRNA及蛋白质的表达。结果DN组大鼠体重、血糖、尿白蛋白,肾脏VEGFmRNA及蛋白质的表达,肾小球细胞数,细胞外基质(ECM)均高于对照组(P<0.01)。与DN组相比,1,25-(OH)2D3组大鼠体重、血糖、尿白蛋白,肾脏VEGFmRNA及蛋白质的表达,肾小球细胞数,ECM聚积显著降低(P<0.01或P<0.05)。尿VEGF与尿白蛋白呈正相关(rs=0.41,P=0.037),与体重、血糖、血肌酐无相关性(rs分别为0.18,0.13,0.21,P>0.05)。结论1,25-(OH)2D3可减轻DN大鼠尿白蛋白的排泄,抑制肾小球系膜增殖,降低VEGFmRNA及蛋白质的表达。Objective To investigate the effects of 1,25-（OH）2D3 on the mRNA and protein expressions of vascular endothelial growth factor （VEGF） in renal tissues from rats of diabetic nephropathy. Methods Thirty-six SD rats were divided into control group, diabetic nephropathy （DN） group, 1,25-（OH）2D3 group equally. DN was induced by peritoneal injection of streptozotocin （STZ）. If the amount of urine albumin excretion （UAE） exceeds 30 rag/24 h, DN is made in rats. 1,25-（OH）2D3 group was made by subcutaneous injection of 1,25-（OH）2D3 to DN rats, once daily for 12 weeks. The body weight, blood glucose, serum creatinine, and 24-hour total urinary albumin were measured. The mRNA and protein expressions of VEGF were determined by reverse transcription-polyrnerase chain reaction （RT-PCR）. Results The body weight, blood glucose, urinary albumin, the mRNA and protein expression of VEGF, the number of glomerular cells and extracellular matrix （ECM） increased significantly in DN rats as compared to those in control （P〈0. 01 ）, and the indexes mentioned above decreased significantly in 1,25-（OH）2D3-treated rats as compared to those in DN rats （P〈0. 01 or P〈0. 05）. Urinary VEGF level was found positively correlated with urinary albumin （rs = 0. 41, P = 0. 037）, but not related to body weight, blood glucose, or creatinine （rs = 0. 18, 0. 13, 0. 21, P〉0. 05）. Conclusion The overexpression of VEGF may participate in the pathogenesis of diabetic nephropathy. 1,25-（OH）2D3 decreases urinary albumin excrection, inhibits the proliferation of glomerular mesangium and downregulates the mRNA and protein ex- pressions of VEGF.

关 键 词：糖尿病肾病 1 25-(OH)2D3 血管内皮生长因子 模型 动物 

分 类 号：R977.24[医药卫生—药品]