RP-HPLC法测定人血浆中盐酸多柔比星的浓度  

Determination of Doxorubicin Hydrochloride Concentration in Human Plasma by RP-HPLC

作　　者：刘一[1] 边原[1] 叶云[1] 

机构地区：[1]泸州医学院附属医院药剂科,泸州市646000

出　　处：《中国药房》2008年第8期598-600,共3页China Pharmacy

摘　　要：目的:建立反相高效液相色谱法测定人血浆中盐酸多柔比星浓度的方法。方法:色谱柱为TianheKromasilC18,流动相为甲醇-0·01mol·L-1磷酸二氢铵溶液-冰醋酸(30:20:0·1),检测波长为233nm,流速为1·0mL·min-1,进样量为20μL,柱温为35℃,内标为柔红霉素。结果:盐酸多柔比星血药浓度在0·25～20·16μg·mL-1范围内与峰面积呈良好的线性关系(r=0·9996);平均方法回收率为96·45%(RSD=2·16%)。结论:本方法简便可行、重现性好,结果准确可靠,可用于临床患者血药浓度监测。OBJECTIVE：To establish an RP-HPLC method for the determination of doxorubicin hydrochloride concentration in human plasma, METHODS： Tianhe Kromasil C18 was used as column, A solution of methenol -0.01 mol·L^-1 ammonium dihydrogen phosphate-acetic acid（30 ： 20 ： 0.1） was used as the mobile phase at a flow rate of 1.0 mL·min^-1.The detection of wavelength was set at 233 nm,The sample size was 20μL and the column temperature was 35℃ .The internal standard was daunorubiein. RESULTS： The calibration curve was linear in the range from 0.25-20.16 μg·mL^-1（r = 0.999 6） ;the average methodological recovery was 96.45% （RSD = 2.16% ） .CONCLUSIONS： The method is rapid, accurate, reproducible and easy to use in clinical detection of blood concentration.

关 键 词：反相高效液相色谱法 盐酸多柔比星 血药浓度 

分 类 号：R969.1[医药卫生—药理学]