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机构地区:[1]华中农业大学农业部猪遗传育种重点开放实验室,武汉430070
出 处:《畜牧兽医学报》2008年第3期268-272,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA
基 金:"863"国家高技术研究发展计划(2006AA10Z1D6);"973"国家重大基础研究项目(2006CB102102)
摘 要:水通道蛋白7(Aquaporin 7,AQP7)是新发现的甘油水孔蛋白,其主要功能是运转甘油。本研究首次获得了大白、长白和梅山猪AQP7基因第4内含子序列,序列比对发现该序列存在G/A突变。采用PCR-PstI-RFLP方法在3个不同猪种之间进行了遗传变异分析,结果表明:大白和长白猪种以G等位基因为主,而梅山猪群以A等位基因为主,对234头大白×梅山F2代资源家系进行性状关联分析,结果表明该位点与猪板油重、内脂率、背膘厚、肥肉率和瘦肥比率等脂肪沉积性状显著相关(P<0.05),且GG基因型个体脂肪沉积性状值显著低于AA基因型个体,提高G等位基因频率可显著减少脂肪沉积。Aquaporin 7 (AQP7) is an newly discovered aquaglyceroprotein and responsible for the movement of glycerol. In this study, we firstly obtained the sequence of intron 4 of the porcine AQP7 gene from Large White, Landrace arid Meishan pig. Sequence comparison revealed a G/A mutation which was detected by PCR-PstI-RFLP. The G allele was predominant in the Large White and Landrace pigs, while there was higher A allele frequency in Meishan pigs. Association analysis between the polymorphism and fat deposition traits was performed in 234 Large white× Meishan Fz pigs. Significant associations of the genotype with fat deposition traits such as leaf fat weight (LFW), internal fat rate (IFR), backfat thickness (BT), fat percentage (FP) and ratio of lean meat versus fat meat (RLF) were found. The individuals with GG genotype have lower fat deposition value than those with AA genotype and allele G was significantly associated with the decreasing of fat deposition.
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