不同繁殖节律母牦牛冷暖季血浆褪黑激素RP-HPLC测定  

Determination of Plasma Melatonin of Female Yaks with Different Reproductive Rhythms by Reversed-phase High-performance Liquid Chromatography in Warm and Cold Seasons

作　　者：文勇立[1] 王永[1] 齐莎日娜[1] 王艳红[1] 马力[1] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041

出　　处：《畜牧兽医学报》2008年第3期302-308,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：四川省应用基础项目"牦牛繁殖节律的分子调控机制研究"(05JY029-037-1);"牦牛主要经济性状生物学基础及分子调控研究"(07JY029-046)

摘　　要：为研究不同季节、不同繁殖节律和繁殖活动期与静止期成年母牦牛血浆MLT分泌水平的差异,应用反相高效液相色谱法(RP-HPLC)检测成年母牦牛血浆MLT浓度。供试母牦牛63头,分为A、B、C 3组,每组21头。A组:1年1胎,繁殖活动期;B组:2年1胎,全奶,繁殖静止期;C组:2年1胎,半奶,繁殖活动期。在暖季的7月21-23日和冷季的11月21-23日,自然光制:12.00L∶12.00D和14.65L∶9.35D,日落前2 h开始采血,持续50min,颈静脉采血每头20 mL,肝素抗凝,离心取血浆。1年1胎母牦牛血浆MLT浓度((68.90±5.82)pg/mL)高于2年1胎全奶个体((44.38±2.61)pg/mL)(P<0.01)和2年1胎半奶个体((57.51±3.82)pg/mL)(P<0.05);母牦牛血浆MLT浓度暖季高于冷季(P<0.05),表明其具有季节波动,并提示除光照外牦牛MLT分泌还与气温等环境因子有关;母牦牛血浆MLT浓度繁殖活动期高于繁殖静止期(P<0.05)。母牦牛MLT分泌存在季节波动,母牦牛季节性繁殖和繁殖节律受到MLT水平的影响。The differences were studied among seasons, reproductive rhythms, breeding active and resting terms. The plasma concentrations of melatonin of adult female yaks were determined by reversed-phase high-performance liquid chromatography. A total of 63 adult female yaks were selected as experimental animals and divided into three groups： A with calving every year and breeding active term, B with calving per two years and full milking and breeding resting term, C with calving per two years and semi-milking and breeding active term. Each group had 21 yaks. In the sampling period of July 21-23 in warm season and November 21-23 in cold season, the yaks were exposed to 12.00 L ： 12.00 D and 14.65 L ： 9.35 D, respectively. Blood samples were collected for 50 minutes starting at 2 h before sunset. A quantity of 20 mL jugular vein blood was drawn from every animal and anticoagulated with heparin sodium. Plasma obtained after centrifugation was estimated for melatonin. The content of plasma melatonin of group A （（68.90-4-5.82） pg/mL） was higher than that of group B （（44. 38 ± 2. 61） pg/mL） （P〈0.01） and group C （（57.51±3.82） pg/mL） （P〈0.05）. The content of plasma melatonin in cold season was lower than that in warm season （P〈0. 05）. The level of plasma melatonin of yak cows in reproductive active term was higher than that of it in fallow term （P〈0.05）. There was a seasonal rhythm in the secretion of plasma melatonin of yak cows. The concentration of plasma melatonin had an effect on the seasonal breeding and reproductive rhythms of female yaks.

关 键 词：牦牛 褪黑激素 反相高效液相色谱法 季节性繁殖 繁殖节律 

分 类 号：S823.85[农业科学—畜牧学] S814.1[农业科学—畜牧兽医]