中药有效部位组方对高温刺激小鼠肠道淋巴细胞体外增殖的影响  

Effect of Chinese Medicine Effective Component Prescription on in vitro Proliferation of Mouse Intestinal Lymphocyte after Stimulation with High Temperature

作　　者：王自力[1] 董虹[1] 刘凤华[2] 毛帅[2] 刘仁志[1] 许剑琴[1] 

机构地区：[1]中国农业大学动物医学院 [2]北京农学院动物科学系,北京102206

出　　处：《畜牧兽医学报》2008年第3期360-364,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：北京市自然科学基金;北京市教委资助重点课题(KZ200310020007);北京市科委攻关课题(Y0705003041631);国家自然科学基金项目(3077156630771591)

摘　　要：根据中兽医"君、臣、佐、使"的组方原则和正交试验设计法将藿香、苍术、黄柏和石膏4味中药的有效部位按照不同剂量组合成8个不同中药有效部位复方,利用MTT法检测高温刺激小鼠肠道淋巴细胞增殖情况,探讨各个复方对高温刺激小鼠肠道淋巴细胞体外增殖的影响及其组方机制。结果表明:以中药有效部位复方A1B1C1D1(黄柏生物碱、藿香挥发油、苍术挥发油各200μg/mL,石膏水提物100μg/mL)对高温刺激小鼠肠道淋巴细胞体外增殖效果最优,复方中以藿香挥发油和黄柏生物碱为主药,苍术挥发油和石膏水提物为臣、佐药,以藿香挥发油和苍术挥发油交互作用较好。The effective components of herba agastachis, rhizoma atractylodis, gypsum fibrosum and cortex phellodendri were composed in different combination according to the principal of traditional Chinese composing prescription and orthogonal design to form 8 prescriptions. The effects of different prescriptions on in vitro proliferation of mouse intestinal lymphocyte after stimulation with high temperature were studied. The results showed that herba agastachis aetherolea and cortex phellodendri alkaloid were the major drugs in the prescription, rhizoma atractylodis aetherolea and gypsum fibrosurn water extract were adjuvant drug and messenger drug. The prescription composed of 200 μg/mL herba agastachis aetherolea, cortex phellodendri alkaloid and rhizoma atractylodis aetherolea, 100 μg/mL gypsum fibrosum water extract, has the best effect on the proliferation of intestinal lymphocyte, and the interaction of herba agastachis and rhizoma atractylodis aetherolea was better.

关 键 词：中药有效部位 正交设计法 高温刺激 淋巴细胞增殖 组方机制 

分 类 号：S853.7[农业科学—临床兽医学]