免疫组化法检测猪圆环病毒2型在人工感染猪体内的分布  

Detection of Distribution of Porcine Circovirus Type 2 in Experimentally Infected Piglets with Immunohistochemical Method

作　　者：唐宁[1] 吕艳丽[1] 郭鑫[1] 杨汉春[1] 赖婷[1] 冯华兵[1] 

机构地区：[1]中国农业大学动物医学院农业部预防兽医学重点开放实验室,北京100094

出　　处：《畜牧兽医学报》2008年第3期372-375,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：北京市自然科学基金(6042017)

摘　　要：将猪圆环病毒2型(PCV2)BF株经滴鼻接种28日龄健康普通仔猪,于接种后不同时间剖杀,采集相关的器官组织制备组织切片,使用免疫组化方法检测病毒在猪体内的分布。结果显示,淋巴结、脾脏、扁桃体等淋巴组织均出现阳性信号,心、肝、肺、肾、胃、十二指肠、大脑及小脑同样存在阳性细胞,其他组织呈阴性反应。阳性信号主要位于感染细胞的胞质中,偶尔出现在细胞核内。结果表明,PCV2确已造成猪体组织感染。28-day-old healthy conventional piglets were inoculated nasally with porcine circovirus type 2 （PCV2） BF strain, the piglets were necropsy on different day post inoculation. Tissues were collected for preparing tissue sections and immunohistochemistry protocol were developed to detect distribution of PCV2 antigens in tissue samples. Results showed that in lymphoid tissues such as lymph node, spleen and tonsil were positive for PCV2 antigen. PCV2 positive signals also presented in heart, liver, lung, kidney, stomach, duodenum, cerebrum and cerebellum. Other tissues were negative for PCV2 antigen. Viral antigens were localized predominately in the cytoplasm of the infected cells and rarely observed in the nucleus of the cells. Results indicated that infection indeed presented in the tissues of the experimental piglets.

关 键 词：猪圆环病毒2型 免疫组化 分布 

分 类 号：S858.285.3[农业科学—临床兽医学]