肺纤维化大鼠肺组织STAT1与血小板源性生长因子表达的研究  

The expressions of STAT1 and PDGF in lung tissue of pulmonary fibrosis rats induced by bleomycin

作　　者：刘金苹[1] 翟乃亮[2] 范贤明[3] 李淑翠[1] 张树平[1] 

机构地区：[1]滨州医学院药理学教研室,滨州市256603 [2]滨州医学院附属医院呼吸内科 [3]四川省泸州医学院附属医院呼吸内科

出　　处：《滨州医学院学报》2008年第1期16-18,共3页Journal of Binzhou Medical University

摘　　要：目的观察肺纤维化大鼠肺组织信号转导和转录活化因子1(STAT1)和血小板源性生长因子(PDGF)表达的变化,探讨其在肺纤维化的作用机制。方法30只Wistar大鼠随机分为博莱霉素组(BLM组)和对照组(NS组),①BLM组:气管内灌注BLM(5mg/kg)诱导肺纤维化;②NS组:气管内灌注NS(剂量与用法同上)。于气管内灌注后第7、14和28天各分别处死5只大鼠,右肺支气管肺泡灌洗(BAL),进行细胞分类、计数;肺组织免疫组化测定STAT1和PDGF蛋白的表达。结果BLM组肺组织中STAT1和PDGF蛋白表达在第7天达高峰,之后下降,第28天时仍高于NS组(P<0.05),而且与支气管肺泡灌洗液(BALF)中的细胞总数密切相关(r值分别为0.892和0.880,P均<0.01)。结论肺纤维化发生的可能机制与肺组织STAT1和PDGF的过度表达有关。Objectives To study the expressions of STAT1 and PDGF in lung tissue of pulmonary fibrosis model induced by bleomycin in rats, and discuss the possible mechanism. Methods Thirty Wistar rats were randomly divided into two groups （ 15 in each group） , they were bleomycin group （BLM group） and control group （NS group）. ①The BLM group was intratracheally instilled with belomycin （BLM） （5mg/kg） ;② NS group was intratracheally instilled with saline （5mg/kg）. Five rats in each group were sacrificed at 7,14,and 28 days after intratrachel instillation. The bronchoalveolar lavage fluid （BALF） of right lung was gotten and the cells counting as well as differentiation was figured out. Lung expressions of STAT1 and PDGF protein were assessed by immunohistochemistry. Results The expressions of STAT1 and PDGF protein in BLM group were the highest at 7th and still elevated over NS group at day 28. The expressions were correlated with the counting of cells, respectively （ r = 0. 892 and 0. 902, P 〈 0. 01 ）. Conclusion The hyperexpressions of STAT1 and PDGF protein in lung may be the mechanism of pulmonary fibrosis.

关 键 词：肺纤维化 博莱霉素 信号转导和转录活化因子 血小板源性生长因子 

分 类 号：R392.1[医药卫生—免疫学]