核苷酸、聚核苷酸和核酸猝灭Tb^(3+)-钛铁试剂络合物荧光的机理研究  

Studies on the Mechanism of Fluorescence Quenching of Tb^(3+)-Tiron Complex by Nucleotides, Polynucleotides and Nucleic Acids

作　　者：赵一兵[1] 王冬媛[1] 郭祥群[1] 许金钩[1] 

机构地区：[1]厦门大学化学系,厦门361005

出　　处：《高等学校化学学报》1997年第5期691-695,共5页Chemical Journal of Chinese Universities

基　　金：国家教育委员会博士点基金

摘　　要：研究了核苷酸、聚核苷酸和核酸对Tb3+－钛铁试剂（TR）络合物的荧光碎灭机理，认为荧光猝灭过程是核苷酸、聚核苷酸和核酸分子中的磷酸基组分与TR竞争Tb3+离子，生成实验条件下无荧光的二元络合物的静态猝灭过程；用Tb3+－TR络合物荧光探针研究DNA嵌入剂和金属离子与DNA相互作用的实验结果说明这一机理是合理的．The mechanism of fluorescence quenching of Tb3+-Trion complex by nucleotides,polynucleotides and nucleic acids has been studied. It was considered that it is a static quenching which is based on the phosphate moieties of nucleotides, polynucleotides and nucleic acids conpeting the Tb3+ ion with Tiron and forming non-fluorescent binary complex under the optimal conditions. The experimental results of nucleic acids reacting with intercalating reagent of DNA or metal ion using Tb3+-Trion complex as a fluorescence probe indicated that the mechanism is reasonable.

关 键 词：核苷酸 聚核苷酸 核酸 荧光猝灭 铽 荧光探针 

分 类 号：Q524.03[生物学—生物化学]