耐热嗜盐菌的分离及16S rRNA基因序列分析  

Isolation and 16S rRNA Gene Sequence Analysis of a Thermotolerant Halophilic Bacterium

作　　者：谢莹[1] 林连兵[1] 季秀玲[1] 魏云林[1] 

机构地区：[1]昆明理工大学生物工程技术研究中心,昆明650224

出　　处：《微生物学通报》2008年第2期166-170,共5页Microbiology China

基　　金：教育部留学回国人员科研启动基金

摘　　要：从位于西藏自治区澜沧江边一个47℃的盐井中分离筛选到一株耐热嗜盐菌菌株YJ0238,对其进行了生理生化特性研究,采用PCR方法扩增其16S rRNA基因序列,并进行了测定。基于生理生化特性和16S rRNA基因序列的同源性比较,以及系统发育分析,发现菌株YJ0238是Idiomarina属中成员zobellii的一个亚种,其16S rRNA基因序列已被GenBank数据库收录,序列号为EF693953。迄今为止,国内极少有关高温、高盐环境中微生物研究的报道,本研究可为今后研究同类极端环境中新的物种资源以及微生物多样性提供素材和参考。A strain of thermotolerant halophilic bacteria YJ0238 was isolated from the salt well at 47℃ in the Kangning countryside where was located at the side of Lancang River in the Tibet Autonomous Region. Some physiological and biochemical properties were characterized. 16S rRNA gene sequence of YJ0238 was amplified by PCR, and its nucleotide sequence was determined. Based on it＇s physiological and biochemical properties, homology and phylogenetic analysis of 16S rRNA gene sequence, strain YJ0238 was identified as a subspecies of the species ldiomarina zobellii. The GenBank accession number of the 16S rRNA gene sequence of strain YJ0238 is EF693953. Until now, there were few reports on the study of high-temperature and high-salt microbial in domestics. The results of this study will provide research material and information for further studies in this area.

关 键 词：耐热嗜盐菌 16S RRNA基因序列 系统发育 

分 类 号：Q93[生物学—微生物学]