基于多光子激发荧光-毛细管电泳的DNA分析新技术  

New Technology for DNA Analysis Based on Multiphoton Excited Fluorescence-capillary Electrophoresis

作　　者：陈胜[1] 徐友志[1] 杜伟[1] 刘笔锋[1] 

机构地区：[1]华中科技大学生物医学光子学教育部重点实验室

出　　处：《光子学报》2008年第2期356-359,共4页Acta Photonica Sinica

基　　金：国家自然科学基金(20405006、20105004);华中科技大学研究生创新基金资助

摘　　要：研制了一套新型毛细管电泳-连续光多光子激发荧光检测系统,并用该系统对含有多种荧光染料的样品进行了电泳分离和多光子激发荧光检测.结果表明:该系统具有分离效率高、质量检测限低和宽谱激发等特点,适合生物体系等复杂样品的测量并实现了DNA样品的分离分析.A novel system, capillary electrophoresis （CE） coupled with continuous wave-based multiphoton excited fluorescence （MPEF） detection, was demonstrated for biomedical analysis. A model sample containing various fluorescent dyestuffs was separated using CE and detected with this unique MPEF detector. It is shown that this CE MPEF system exhibites various advantages such as high performance separation capability, low mass detectability and spectral excitability in a wide rang. And this novel system is employed successfully for DNA analysis, which indicated a promising future of CE-MPEF method in biomedical analysis.

关 键 词：毛细管电泳 多光子激发荧光 DNA分析 

分 类 号：R318.5[医药卫生—生物医学工程]