利用甲硝唑及外加氧方法筛选耐氧产氢Klebsiella oxytoca HP1 突变菌株  

Screen of O_2-tolerate Phenotype of Klebsiella oxytoca HP1 Mutants with High H_2-evolving Activity by Selection with MNZ Combination O_2

作　　者：邬小兵[1] 徐惠娟[1] 支小鹏[1] 徐方成[2] 胡忠[1] 龙敏南[1] 

机构地区：[1]厦门大学生命科学学院,厦门 361005 [2]厦门大学化学化工学院,厦门 361005

出　　处：《微生物学通报》2008年第3期327-331,共5页Microbiology China

基　　金：福建省青年科技人才创新项目(No.12005J003);厦门市科技项目(No.13502Z20041070)

摘　　要：氢酶是生物制氢的关键酶,大多数氢酶因对氧极敏感而易失活,因此提高氢酶的氧耐受性对生物制氢有重要意义。本研究利用1%甲基磺酸乙酯对Klebsiella oxytoca HP1进行了两轮诱变,经40mmol/L甲硝唑和21%氧联合处理1h(第一轮诱变)或2h(第二轮诱变)进行筛选。所得突变菌株经产氢测试,结果在15%氧浓度条件下,第一代突变菌株HP1-A15产氢活性为出发菌株Klebsiella oxytoca HP1的3.70倍,在21%氧浓度条件下第二代突变菌株HPA15-37产氢活性为HP1-A15菌株的2.75倍,是出发菌株的11倍。突变菌株HP1-A15和HPA15-37具有较好的遗传稳定性。本试验结果说明利用MNZ和外加氧的方法适用于兼性厌氧菌耐氧产氢突变菌株的筛选。Hydrogenases are key enzyme for bio-hydrogen production, most of them were rapidly inactivated by oxygen. It is important to bio-hydrogen production and hydrogen application that improve the O2-tolerance of hydrogenase. In this experiment, the hydrogen producing strain Klebsiella oxytoca HP1 was treated with 1% ethyl methanesulfonate（EMS） , the mutants with high O2-toleration ability were screened with 40mmol/L metronidazole （MNZ） and 21% oxygen. The H2-evolving activity of the first generation mutant HP1-A15 was increased 3.70 times than that of the wild-type （WT） under 15% oxygen. The H2-evolving activity of the second generation mutant HPA15-37 was enhanced 11 times than that of WT under the condition of 21% oxygen. The mutants HP1-A15 and HPA15-37 had steady heredity. These results suggest that MNZ and in addition oxygen is a good way to screen of O2-tolerate phenotype of facultativeanaerobe with high H2-evolving activity.

关 键 词：氢酶 耐氧 甲硝唑 突变 KLEBSIELLA oxytoca HPL 

分 类 号：Q93[生物学—微生物学]