丹黄方对大鼠肝再生过程中PC3、c-fos、LRF-1影响的实验研究  

The experiment research of Danhuangfang influence expression of PC3 mRNA,c-fos mRNA,LRF-1 mRNA in rat liver regenerative process

作　　者：朱清静 杨玲[2] 黎运呈[3] 陈锋[4] 蔡艳萍 周星 阮连国 李霞 万十千 张巍 李传龙 盛国光[3] 

机构地区：[1]武汉市传染病医院中西医结合重点专科,湖北武汉430022 [2]华中科技大学同济医学院附属协和医院 [3]湖北省中医院 [4]华中科技大学同济医学院

出　　处：《中西医结合肝病杂志》2008年第1期32-34,37,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：国家中医药管理局中医药科学技术研究专项(No.06-07JP25);湖北省科技厅科技攻关计划项目(No.2005AA301C12)

摘　　要：目的:探讨丹黄方对大鼠肝切除肝再生模型中促肝细胞再生的作用机制。方法:采用肝部分切除肝再生模型,用丹黄方进行干预,通过RT-PCR、电泳凝胶等方法检测与肝再生密切相关因子PC3 mRNA、c-fos mRNA、LRF-1 mRNA的表达,观察丹黄方对肝细胞再生的影响。结果:丹黄方对肝再生模型大鼠肝组织PC3 mRNA、c-fos mRNA的表达有增强作用,与模型比较,差异有显著性意义;对肝组织LRF-1 mRNA表达的影响不显著。结论:丹黄方可能是通过增强肝组织PC3 mRNA、c-fos mRNA的表达而促进肝细胞的增殖。To approach the function mechanism of Danhuangfang （DHF） promoting the liver cell regenerate on the liver partial excision of liver regeneration model in rats. Methods： To adopt the liver partial excision of liver regeneration model in rats, carry on the intervention with DHF, examine the correlation factor which close liver regeneration expression of PC3 mRNA, c-fos mRNA, LRF-1 mRNA by gel electrophoresis and RT-PCR, observe the effectiveness which DHF influence liver regeneration. Results： DHF could enhance the hepatic tissue expression of PC3 mRNA, c-fos mRNA, LRF-1 mRNA in rats liver regeneration model, the DHF group had the significance difference comparing with the model group ; the DHF effected the hepatic tissue expression of LRF-1 mRNA which not to be remarkable. Conclusion： DHF can possibly promote the liver cell multiplication through enhancing the hepatic tissue expression of PC3 mRNA, c-fos mRNA.

关 键 词：丹黄方 肝再生 抗增殖相关分泌蛋白 细胞癌基因fos 肝再生因子-1 

分 类 号：R285.5[医药卫生—中药学]