放疗后早期肿瘤组织内^99Tc^m-rh-Annexin V分布与Survivin、Caspase-3蛋白表达的相关性研究  

Distribution of ^99Tc^m-rh-Annexin V and Its Relationship With Expression of Survivin, Caspase-3 in Tumor Early After Radiotherapy

作　　者：张欣[1] 李亚明[2] 张延军[1] 季晓鹏[3] 陶莉[1] 张剑英[4] 赵贞贞[4] 朱毅[1] 杨春[1] 赵明[3] 田爱娟[3] 

机构地区：[1]大连医科大学附属第一医院核医学科,辽宁大连116011 [2]中国医科大学附属第一医院核医学科,辽宁沈阳110001 [3]大连医科大学附属第二医院核医学科,辽宁大连116023 [4]北京师范大学化学学院,放射性药物教育部重点实验室,北京100875

出　　处：《同位素》2008年第1期29-35,共7页Journal of Isotopes

基　　金：辽宁省教育厅科学研究项目（20060204）

摘　　要：采用^99Tc^m-rh-Annexin V作为凋亡显像示踪剂，观察小鼠放疗后早期肿瘤组织内Survivin、Caspase-3蛋白表达。结果显示，放疗组肿瘤组织内^99Tc^m-rh-Annexin V分布、TUNEL检测阳性细胞数及Caspase-3蛋白表达均明显多于对照组，Survivin蛋白表达A组明显高于B组，差异均存在显著性（P均＜0．01）。相关性研究表明，肿瘤组织的放射性摄取与TUNEL阳性细胞数呈明显正相关（r=0．942，P=0．000）；肿瘤组织内Survivin蛋白表达与Caspase-3蛋白表达呈明显负相关（r=-0．836，P=0．000）。肿瘤组织内^99Tc^m-Annex-inV分布与Caspase-3蛋白表达呈明显正相关（r=0．948，P=0．000），与Survivin蛋白表达呈明显负相关（r=-0．819，P〈0．01）。以上结果提示，肿瘤组织内^99Tcm—rh—Annexin V的分布可以反映放疗后早期肿瘤组织细胞凋亡的状况以及凋亡调控蛋白Survivin、Caspase-3表达水平的变化。The early expression of Survivin and Caspase-3 in tumor cells after radiotherapy with ^99Tc^m-rh-annexin V as the tracer of apoptosis imaging was studied. Tumor cell apoptosis was examined by TUNEL methods, and the expressions of Survivin and Caspase-3 in tumor cells was determined with immunohistochemical methods. Radiotherapy significantly increased the tumor uptake of ^99Tc^m-rh-annexinV and the positive number of TUNEL, as well as the expression of Caspase-3 （P〈0. 010）. The expression of Survivin was significantly high in contrast group than in treated group （P〈0. 010）. The distribution in tumor correlated well with the positive number of TUNEL in contrast group and treated group （r =0. 942, P=0.000）. The expression of Survivin correlated negatively with the expression of Caspase-3 the two group （r=--0. 836, P=0. 000）. The accumulation of 99Tcm-rh-an- nexinV in tumor correlated positively with the expression of Caspase-3 （r=0. 948, P=0. 000）, and correlated negatively with the expression of Survivin （r=-0. 819, P=0. 000）. The distribution of ^99Tc^m-rh-annexin V can not only truly reflected the degree of apoptosis in tumor, but also the change of survivin and caspase-3 expression early after radiotherapy.

关 键 词：^99Tc^m-rh-Annexin V SURVIVIN CASPASE-3 表达 相关性 

分 类 号：Q691.4[生物学—生物物理学] R817.1[医药卫生—影像医学与核医学]