粒细胞集落刺激因子促进自体骨髓单个核细胞向急性肝损伤小鼠肝脏迁移的实验研究  

Effects of autologous bone marrow mononuclear cell mobilization with granulocyte colony-stimulating factor for the therapy of acute liver failure in mice

作　　者：金世柱[1] 韩明子[1] 孙立影[1] 耿莹[1] 洪钰[1] 

机构地区：[1]哈尔滨医科大学附属第二医院消化内科,黑龙江哈尔滨150086

出　　处：《胃肠病学和肝病学杂志》2008年第3期230-233,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：黑龙江省自然科学基金(D200536)

摘　　要：目的探索粒细胞集落刺激因子促进自体骨髓单个核细胞向急性肝损伤小鼠肝脏迁移的作用。方法以红色荧光染料PKH26标记从小鼠骨髓中分离出的骨髓单个核细胞,从小鼠的尾静脉注入同种异体的CCL4-AAF造成肝损伤的小鼠体内,移植2周后取肝组织,通过荧光显微镜观察实验组及对照组小鼠骨髓干细胞向肝脏迁移的情况。结果两组小鼠的肝小叶中央静脉及汇管区均可见新生的肝细胞,PKH26标记阳性的细胞在实验组20倍镜下每张切片平均为(102.76±37.304)个,在对照组平均(53.84±29.987)个(P<0.05)。结论重组粒细胞集落刺激因子可以促进骨髓单个核细胞向急性肝损伤小鼠的肝脏迁移。Objective To explore the amount of bone marrow mononuclear cells migrating to the liver during allografting in mice. Methods Bone marrow mononuclear cells were isolated from the bone marrow of mice by lymphocyte separation medium and labeled with PKH26. The labeled cells were subsequently infused into the caudal veins of different mice with homogeneity, in which hepatic injury was induced by carbon tetrachloride and 2-acetylminofluorene. Two weeks after transplantation, the migration status of the cells were studied by fluorescence microscopy. Results Liver cells were found at portal region and central veins of hepatic lobules in all the mice. The PKH26 labeled cells appeared at an average frequency of 102.76 ± 37. 304 in the test group, and at an average frequency of 53.84 ± 29. 987 in the control group. There was significant difference between them （P 〈 0.05）. Conclusion G-CSF can promote migration of BMMC to the liver in mice with acute liver failure.

关 键 词：粒细胞集落刺激因子 骨髓单个核细胞 急性肝损伤 自体 

分 类 号：Q813[生物学—生物工程]