纳米蜂胶对肿瘤细胞K562，L929，MNK28的杀伤效应及机理研究  

Anti-tumor Effect of Nano-propolis on L929,MNK28 and K562 Cell Lines

作　　者：杨道锋[1] 朱慧芬[2] 邵静芳[2] 张悦[2] 沈关心[2] 龚非力[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,武汉430030 [2]华中科技大学同济医学院免疫学教研室

出　　处：《中国药师》2008年第3期269-272,共4页China Pharmacist

摘　　要：目的:了解纳米蜂胶对常见肿瘤细胞的体外杀伤效应,并探讨其机理。方法:乳酸脱氢酶法测定纳米蜂胶体外对小鼠成纤维瘤细胞、人胃癌细胞、人红白血病细胞和非肿瘤源性的脐静脉内皮细胞的杀伤作用。流式细胞仪测定细胞周期和凋亡率。结果:纳米蜂胶在浓度16～400μg·ml^(-1)范围内对3种肿瘤细胞都有一定的杀伤作用,且随着浓度的升高杀伤率增加,其对肿瘤细胞的杀伤作用明显强于普通蜂胶,但低于氟尿嘧啶。纳米蜂胶对K562,L929,MNK28半数致死浓度(IC_(50))分别为(357.8±8.3)μg·ml^(-1),(472.3±27.5)μg·m^(-1),(461.7±18.7)μg·ml^(-1)。而普通蜂胶对K562,L929和MNK28的IC50分别为(737.6±74.3)μg·ml^(-1),(753.6±82.1)μg·ml^(-1),(939.3±75.5)μg·ml^(-1)。而两种蜂胶对人脐静脉内皮细胞的毒性均较低。细胞凋亡率与杀伤率有一定的线形关系,纳米蜂胶作用后处于G_0/G_1期的肿瘤细胞的比例明显增高。结论:纳米蜂胶体外对小鼠成纤维瘤细胞、人胃癌细胞、人红白血病细胞具有明显的杀伤作用,诱导肿瘤细胞凋亡是其抗肿瘤的机制之一。Objective： To investigate the anti-tumor effects of general propolis（GP） and nano-propolis （NP） on K562 cell line, L929 cell line, MNK28 cell line, and their impact for cell cycle and apoptosis. Method： A consecutive concentrations of GP and NP were cultured with K562, L929, MNK28 cells, respectively. HuVEC cells were employed as a control. Cell death rates were detected by Iactate dehydrogenase method. Apoptosis and cell phases were detected by flow cytometry. Result： The GP and NP at 16 -400 μg·ml^-1 displayed significant killing activity on K562, L929 and MNK28 cells, the killing activities were concentration dependent. The IC50 of NP for K562, L929, MNK28 cells were 357.8 ± 8.3 μg·ml^-1,472.3 ±27.5 μg·ml^-1, 461.7 ± 18.7 μg·ml^-1, respectively; that of GP were 737.6 ± 74.3 μg·ml^-1,753.6 ± 82.1 μg·ml^-1,939.3 ± 75.5μg·ml^-1, respectively. Both GP and NP did not displayed an obvious killing effect on HuVEC, a non-tumor cell. Flow cytometry showed that apoptosis rates of tumor cells was increased as their killing rates increased, most of tumor cells were stopped at G0/G1 phase after treatment of nono-propolis. Conclusion： Nano-propolis can kill K562, L929 and MNK28 cells effectively, the part of their antitumor effeciacy is mediated by the induction of apoptosis.

关 键 词：纳米蜂胶 蜂胶 肿瘤细胞 杀伤率 

分 类 号：R965.1[医药卫生—药理学]