去细胞结合光氧化技术处理牛颈静脉抗钙化性能研究  

The anti-calcification properties of BJVC treated with dye-mediated photooxidation following decellularization

作　　者：李伟[3] 吴忠仕[1] 杨丽娟[2] 刘哲亮[1] 胡建国[1] 刘文亮[1] 胡野荣[1] 

机构地区：[1]中南大学湘雅二医院心胸外科,长沙410011 [2]滨州医学院生理教研室 [3]山东滨州医学院附属医院胸心外科,256602

出　　处：《中华胸心血管外科杂志》2008年第1期39-41,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：本课题受湖南省科技计划重大专项基金（04SK1005）、湖南省自然科学基金（06JJ50069）资助 致谢：感谢中南大学湘雅二医院心胸外科李小冰、乔刚、张明、吕卫东、赵向东、吴坤等研究生同学在本实验中给予的支持与帮助

摘　　要：目的探讨去细胞结合光氧化技术处理后的牛颈静脉带瓣管道（BJVC）在体内的抗钙化性能。方法取新鲜牛颈静脉20根，每根裁出4个带瓣血管片，随机分为A、B、C、D4组，分别以染料介导光氧化、戊二醛、去细胞及去细胞结合光氧化4种技术处理。建立大鼠皮下包埋模型，2个月后获取组织标本，原子吸收光谱法测定组织钙含量；并应用X线、CT、骨密度扫描检测整体组织钙化情况。结果（1）D组试片标本形态较完整，柔韧性好，无结节样改变；其整体组织钙盐密度明显低于A组及B组，与C组接近。（2）D组管壁组织钙含量显著低于A组及B组（P〈0．01），与C组差异无统计学意义（P〉0．05）；D组瓣膜组织的钙含量显著低于B组（P〈0．01），与A组及C组比较均差异无统计学意义（P〉0．05）。结论去细胞结合光氧化技术与传统戊二醛及染料介导光氧化技术比较，能明显提高牛颈静脉体内抗钙化性能。Objective To study the anti-calcification properties of BJVC treated by dye-mediated photooxidation following decellularization in vivo. Methods Each of twenty fresh bovine jugular veins with a retained native valve procured from a slsughter- house was cut into 4 trial patches with valve, which were randomly divided into group A, B, C and D, which were treated respectively by the technology of dye-mediated photooxidation, glutaraldehyde, decellularization and dye-mediated photooxidantion following decel- lularization. One of each group trial patches were implanted subcutaneously in the same Wistar rat. 60 days after implantation, all trial rats were sacrificed and the specimens were retrieved. The tissue calcium content was determined by flame atomic absorption spectrophotometer. Tne tissue calcium salt distribution was evaluated by X-my, spiral computed tomography, bone density scanning. Results （ 1 ） The trial patches in D group were relative integrity, soft and tenacious, no nodes emerged,its calcium salts density in total was significantly less than those of group A and B, and was similar to group C. （2）The calcium content of vessel wall in group D was significantly lower than those of group A and B （ P 〈 0.01 ）, and was no different with which of group （ P 〉 0.05）. The calcium content in the valves in group D was lower than that of group B （ P 〈 0.01）, and was no different with those of group A and D（ P 〉 0.05）. The technology of dye-mediated photooxidation following decellularization can effectively prevent the calcification of bovine jugular venous conduit in vivo.

关 键 词：生物假体 钙化 生理性 颈静脉牛 氧化剂 光化学 去细胞 光氧化 

分 类 号：R686[医药卫生—骨科学]