落叶松种间及无性系间ISSR鉴别技术的研究  

Research on ISSR Identification Technology of Larch Species and Clones

作　　者：张磊[1] 张含国[1] 李雪峰[1] 姜静[1] 魏志刚[1] 

机构地区：[1]北林业大学林木遗传育种与生物技术教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2008年第2期216-221,共6页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(30640034);国家林业局重点项目(2006-37);国家科技支撑项目(2006BAD01A1401-01)

摘　　要：采用ISSR分子标记技术对兴安、长白和日本落叶松种间以及不同无性系进行了鉴别。从49条引物中筛选出13条ISSR引物可以对落叶松种间和无性系间进行鉴别,特异条带个体的百分率为100%,该项技术为落叶松新品种以及良种的准确鉴别提供了新的途径和手段:其中5条引物在日本落叶松、兴安落叶松和长白落叶松不同位置扩增出特异谱带,作为种的鉴定的标准,有9条引物可以对落叶松种内不同无性系分别扩增出特异片段,进行无性系的鉴别。This study used ISSR molecular marker technology to identify Larix Larix kaempferi and different clones. Thirteen ISSR primers which screened from forty-nine primers can identify among larch species and clones, and the rate of the samples with specific band is 100%, this technology offers new ways and means for accurate identification of new larch species and elite seeds： there are five primers by which L. kaempferi, L. olgensis and L. kaempferi specific bands were produced at different location, as a species identification be amplified specific fragment in different kinds of larch clones respectively. were amplified particularly, standards, nine primers can It can identify the clones.

关 键 词：落叶松 种 无性系 ISSR 鉴别 

分 类 号：S893.1[农业科学—特种经济动物饲养]