利用RT-PCR从人胎肝获得血小板生成素cDNA  

Thrombopoietin (TPO) cDNA obtained by RT-PCR from human fetal liver

作　　者：田生礼[1] 冯彪[1] 刘及[1] 刘丽[2] 

机构地区：[1]白求恩医大预防医学院卫生毒理教研室 [2]解放军空军总医院分子生物学研究中心

出　　处：《白求恩医科大学学报》1997年第5期460-462,共3页Journal of Norman Bethune University of Medical Science

摘　　要：采用反转录-PCR（RT-PCR）技术，成功地从人胎肝细胞mRNA中获得了血小板生成素（TPO）信号肽和成熟肽编码区cDNA。序列分析表明，该cDNA序列与DeSauveg等报道的人TPO相应的核苷酸序列完全一致，为TPO基因工程的进一步研究打下基础。Human thrombopoietin cDNA including signal and mature peptide has been successfully obtained by means of reverse transcription polymerase chain reaction (RT- PCR) from fetal livermRNA. Sequence analysis indicated that the sequence of human TPO cDNA was the same as that ofhuman TPO cDNA reported by De Sauvage et al. It laid foundation for further study of genetic engineering.

关 键 词：血小板生成素 基因克隆 RT-PCR CDNA 胎肝 

分 类 号：Q75[生物学—分子生物学]