高效液相色谱法测定卡络磺钠的有关物质  

Determination of related substances in Carbazochrome Sodium Sulfonate by HPLC

作　　者：胡士高[1] 

机构地区：[1]安徽省药物研究所,安徽230022

出　　处：《安徽卫生职业技术学院学报》2008年第1期80-81,共2页Journal of Anhui Health Vocational & Technical College

摘　　要：目的:建立一种高效液相色谱法测定卡络磺钠的有关物质。方法:用Diamonsil-C18色谱柱(250mm×4.6mm,5μm),0.12%磷酸二氢铵溶液-乙腈(91:9)作为流动相,流速为1.0m1/min,检测波长363nm。结果:卡络磺钠的检测限为0.6ng,卡络磺钠与各杂质峰分离良好。结论:该方法专属性强,可用于卡络磺钠的质量控制。Objective To establish an HPLC method for determination of related substances in Carbazochrome Sodium Sulfonate. Methods The HPLC method was performed on Diamonsil-C18 column （250mm×4.6mm,5μm）, 0.12% ammonium dihydrogen phosphate solution - acetonitrile （91：9）as mobile phase, the flow rate was 1.0ml· min^-1,and detection wavelength was set at 363nm. Results The detection limits for Carbazochrome Sodium Sulfonate was 0.6ng.Good resolution for Carbazochrome Sodium Sulfonate and related substances was obtained.Conclusion The method was specific and can be used for quality control of Carbazochrome Sodium Sulfonate.

关 键 词：卡络磺钠 有关物质 高效液相色谱法 测定 

分 类 号：R917[医药卫生—药物分析学]