提高HPLC检测灵敏度的新方法  

Novel study on improvement sensitivity of HPLC detection

作　　者：魏玉霞[1] 牟晓玲[1] 朱勇[1] 庆宏[1] 邓玉林[1] 

机构地区：[1]北京理工大学生命科学与技术学院

出　　处：《生命科学仪器》2008年第2期44-47,共4页Life Science Instruments

摘　　要：建立了不同固定相导致速度差实现堆积的理论模型,并以2-硝基酚,对羟基苯甲酸甲酯和3-硝基酚为模型化合物,进行了堆积现象研究。结果表明,不同固定相导致的速度差能实现堆积,进而显著提高HPLC的检测灵敏度。在本文考察的最佳堆积条件下,2-硝基酚,对羟基苯甲酸甲酯和3-硝基酚的检出限分别降低了4.5,5.0和9.1倍。An on-column enrichment HPLC procedure for conventional HPLC columns and instrumentation has been theoretically and experimentally established. It involves the on-column stacking resulted from the difference of migration velocity of one compound on different stationary phase and further focusing with a mobile phase of high elution strength. The method was demonstrated by the use of 2-nitrophenol, 3-nitrophenol and methyl p-hydroxybenzoate as model system. Under the optimal on-column enrichment conditions, 4.5 fold sensitivity enhancement was obtained for 3-nitrophenol, 5 fold and 9.1-fold sensitivity enhancement was achieved for 2- nitrophenol and methyl p-hydroxybenzoate, respectively.

关 键 词：速度差 堆积 灵敏度 

分 类 号：R114[医药卫生—卫生毒理学]