野生稻细菌性条斑病抗性资源筛选及遗传分析  

Identification and Genetic Analysis of Resistance to Bacterial Leaf Streak in Wild Rice

作　　者：黄大辉[1] 岑贞陆[2] 黄大辉[3] 刘驰[1] 贺文爱[3] 贺文爱[4] 陈英之[3] 陈英之[4] 马增凤[1] 马增凤[4] 杨朗[2] 杨朗[3] 韦绍丽 韦绍丽[4] 刘亚利[3] 刘亚利[4] 黄思良[2] 黄思良[3] 杨新庆[1] 李容柏[1] 李容柏[3] 李容柏[4] 

机构地区：[1]广西农业科学院水稻研究所,南宁 530007 [2]广西农业科学院植物保护研究所,南宁 530007 [3]广西农科院广西作物遗传改良生物技术重点开放实验室,南宁 530007 [4]广西大学农学院和广西亚热带生物资源保护利用重点实验室,南宁 530005

出　　处：《植物遗传资源学报》2008年第1期11-14,共4页Journal of Plant Genetic Resources

基　　金：广西科技创新能力与条件建设资助项目(桂科能05112001-1A1,桂科能0630006-5B,桂科能0630006-5F)

摘　　要：对1655份普通野生稻(Oryza rufipogon Griff.)和31份药用野生稻(O.officinalis Wall.ex Watt)进行了细菌性条斑病(Xanthomonas oryzae pv.oryzicola,简称细条病)抗性鉴定,结果发现在普通野生稻中有57份抗病材料,其中3级抗性有31份,占总数的1.87%;5级中抗有26份,占总数1.57%。在药用野生稻资源中,有15份抗病材料,占总数的48.4%。选取了8份普通野生稻抗性资源(分别命名为DP1、DP3、DP5、DP9、DP15、DP16、DP17和DP20)与9311杂交,再自交或与9311回交后,分别获得BC1、F1和F2后代。在接种鉴定中发现这些抗性资源的BC1或F1所有植株均对细条病表现感病,说明这8份材料的抗性属于隐性遗传。在DP3与9311杂交的F2群体中,抗感植株的分离比符合1∶15的比例,说明DP3的抗性由2对隐性重叠作用基因控制。研究结果表明,在野生稻中可以获得一批具有较大利用价值的细条病抗性资源,其中药用野生稻资源中抗性材料所占的比例较大。Bacterial leaf streak （ Xanthomonas oryzae pv. oryzicola） is a destructive disease of rice in China. In the present study wild rice originating from Guangxi Province was screened for obtaining resistant resources to the bacterial disease. Among 1655 accessions of Oryza rufipogon Griff., 31 （1.87%） were identified to be resistant. In another screening, 15 （48.4%） out of 31 accessions of O. officinalis Wall. ex Watt were resistant. For genetic analysis of the resistance, 8 resistant accessions of O. rufipogon Griff., named DP1, DP3, DP5, DP9, DP15, DP16, DP17 and DP20, were crossed and back-crossed with indica rice variety 9311. And it was found that all plants of F1 or BC1 progenies inoculated with the pathogen were susceptible to the disease. The results suggested that resistance from these 8 accessions be controlled by recessive genes. Segregation of resistant and susceptible progenies fitted a 3：1 ratio in the F2 population derived from DP3 and 9311. It seemed that the resistance originated from resistant source DP3 was controlled by two pairs of recessive genes in duplicate interaction.

关 键 词：普通野生稻 药用野生稻 细菌性条斑病 抗性资源 遗传分析 

分 类 号：S511.9[农业科学—作物学] S435.111.4