小麦组织培养再生系统及农杆菌介导的转基因技术研究  

Study of Wheat Tissue Culture Regeneration System and Genetic Transformation Mediated by Agrobacterium tumefaciens

作　　者：朱晋云[1] 许玉娟[1] 杨丽萍[1] 亢秀丽[1] 孙毅[2] 杜建忠[2] 

机构地区：[1]山西省农业科学院小麦研究所,临汾 041000 [2]山西省农业科学院生物技术中心,太原 030031

出　　处：《植物遗传资源学报》2008年第1期84-89,共6页Journal of Plant Genetic Resources

基　　金：山西省回国留学人员基金重点项目(97-1-非11);山西省自然科学基金项目(20041086)

摘　　要：通过对晋麦47、鲁麦14、晋阳345、晋麦31等4个小麦品种的组织培养再生系统研究，结果表明，在MS基本培养基中加入Kao＇s有机化合物和T维生素后，小麦幼胚胚性愈伤组织诱导率提高31.4％～42.4％，植株再生能力提高50％～80％。对幼胚愈伤组织进行低温预处理和在农杆菌转化时添加AS处理后，经PPT筛选获得大量正常再生植物，PCR及Southern杂交检测证明其中28株再生植株呈阳性，这些植株明显提高了对basta的抗性。Tissue culture regeneration system of wheat varieties Jinmai 47, Lumai 14, Jinyang 345 and Jinmai 31 were studied. The results indicated that when Kao＇s organic compound and T vitamins were supplemented on MS basic medium, the frequency of embryogenic callus induction increased by 31.4% -42.4%, and frequency of plant regeneration increased by 50%-80%. Wheat culli were pretreated in lower temperature condition and aceto-syringone （AS） were simultaneously put in bacterial liquid medium and culli medium on Agrobacterium tumefaciens-mediated transformation. PPT selection and further PCR and Southern blot test demonstrated that bar gene has been integrated into plant genome.

关 键 词：小麦 愈伤组织 农杆菌 遗传转化 

分 类 号：S512.1[农业科学—作物学] TE966.03[石油与天然气工程—石油机械设备]