阿魏酸哌嗪对单侧输尿管梗阻大鼠肾脏的保护作用  

Protective effect of piperazine ferulate on rat kidney after unilateral ureteral obstruction

作　　者：马爱景[1] 谢席胜[1] 付平[1] 李静[1] 米绪华[1] 冀玲[1] 陶冶[1] 

机构地区：[1]四川大学华西医院肾脏内科,四川成都610041

出　　处：《西部医学》2008年第2期234-236,共3页Medical Journal of West China

基　　金：四川大学华西医院横向科研基金资助(No.H06-011)

摘　　要：目的观察阿魏酸哌嗪(Piperazine ferulate,PF)对单侧输尿管梗阻(Unilateral ureteral obstruction,UUO)模型大鼠肾小管间质的转化生长因子-β1(Transforming growth factor-β1,TGF-β1)、α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)和I型胶原(Collagen I,ColI)表达水平的影响,初步探讨其对小管间质纤维化的作用机制。方法将24只大鼠分为假手术(Sham-operated rats,SOR)组、UUO组、氯沙坦(Angiotensin Ⅱreceptor blocker,ARB)组和PF组。运用免疫组化技术半定量分析实验第14天各组大鼠肾组织中TGF-β1、a-SMA和ColI的表达水平。结果免疫组化结果表明SOR组TGF-β1、a-SMA和ColI均为少量表达,UUO组大鼠上述表达明显增加;ARB组和PF组TGF-β1、a-SMA和ColI的表达均低于UUO组,但高于SOR组,两治疗组之间差异无统计学意义。结论阿魏酸哌嗪可以降低肾小管间质TGF-β1、a-SMA和ColI的表达水平,延缓肾小管间质纤维化的进程。Objective To explore the effect and mechanism of piperazine ferulate on regulating the expression of transforming growth factor-β1 （TGF-β1）, α-smooth muscle actin （α-SMA） and collagen Ⅰ （ColⅠ） in renal tubulointerstitium in the rat model of unilateral ureteral obstruction （UUO） Methods Twenty-four SD rats were randomly divided into sham-operation group, UUO group, angiotensin Ⅱ receptor blocker treatment group, and piperazine ferulate treatment group. The expression levels of TGF-β1,α-SMA and ColⅠ in the rats were determined by immunohistochemistry assay at 14 days of the experiment. Results Immunohistochemistry results showed that there was slight expression of TGF-β1, α-SMA and ColⅠ in sham-operation group, but the expression in UUO group was significantly increased （P〈 0. 05）. The expression of TGF-β1, α-SMA and ColⅠ in angiotensin Ⅱ receptor blocker treatment and piperazine ferulate treatment groups was lower than that in UUO group but higher than that in sham-operation group （P〈0. 05）. No significant difference in the expression levels was found between the two treatment groups（P〉0. 05）. Conclusion Piperazine ferulate can decrease the expression of TGF-β1,α-SMA and ColⅠ, suggesting it can delay the aggravation of renal tubulointerstitial fibrosis.

关 键 词：阿魏酸哌嗪 肾间质纤维化 TGF—β1 Α-SMA ColⅠ 

分 类 号：R693.2[医药卫生—泌尿科学]