铁皮石斛疫病及其病原菌  

The causal agent of Dendrobium candidum blight disease

作　　者：李静[1] 张敬泽[1] 吴晓鹏[1] 单卫星[2] 徐同[1] 

机构地区：[1]浙江大学生物技术研究所,杭州310029 [2]西北农林科技大学植物保护学院,杨凌712100

出　　处：《菌物学报》2008年第2期171-176,共6页Mycosystema

基　　金：2001年国家科技攻关计划引导项目(No.2001BA744C);浙江省自然科学基金(No.Y304071)

摘　　要：铁皮石斛疫病2001年发现在浙江义乌栽培田间,是由疫霉菌引起的。在田间,疫霉菌侵染茎基部,引起当年移植苗根腐、植株枯萎和死亡,但侵染2-3年植株幼嫩顶部仅引起顶枯症状。通过对病原菌形态学、交配型的观察,以及核糖体DNAITS序列分析,侵染铁皮石斛的5个分离菌株被鉴定为烟草疫霉菌Phytophthora nicotianae。致病性试验表明,铁皮石斛是烟草疫霉菌的寄主。Dendrobium candidum blight caused by Phytophthora species was found for the first time in cultivated field in Yiwu city, Zhejiang province of China in 2001. One-year-old infected transplanting seedlings demonstrate typical symptoms of Phytophthora root rot, wilting and death, whereas 2- to 3-year-old infected plants only have die-back symptoms with almost no root and basal stem rot. Morphological observation, matingtypes and DNA sequences analyses proved that five isolates of the causal agent were all P nicotianae. Pathogenicity tests confirmed that D. candidum is a natural host of P nicotianae.

关 键 词：卵菌 鉴定 烟草疫霉菌 铁皮石斛 疫病 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]