没药倍半萜诱导的p21^(WAF/CIP1)蛋白参与抑制前列腺癌细胞增殖  

Overexpression of p21^(WAF/CIP1) is involved in sesquiterpenoids-mediated inhibitory effect on proliferation of prostate cancer cells

作　　者：王小玲[1] 孔峰[1] 吉恺[1] 蔡捷[1] 任凯[1] 龚磊[1] 胡志敏[1] 苑辉卿[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学教研室,山东济南250012

出　　处：《毒理学杂志》2008年第1期10-13,共4页Journal of Toxicology

基　　金：山东省卫生厅重点项目(HZ023);山东省自然科学基金项目(Y2004C24)

摘　　要：目的探讨没药的两个倍半萜单体化合物抑制前列腺癌细胞增殖的作用机制。方法应用细胞增殖实验噻唑蓝(MTT)法检测化合物对人前列腺癌细胞株LNCaP的影响;流式细胞术进一步分析前列腺癌细胞经化合物处理后细胞周期时相的变化;Western blot检测化合物对细胞周期相关蛋白p21WAF/CIP1(p21)和cyclin D表达的影响;利用细胞转染技术检测化合物对cyclin D启动子表达活力的影响。结果两个没药倍半萜单体化合物对前列腺癌细胞均有显著的抑制活力,使细胞停滞于G0/G1期;且能在蛋白水平诱导p21WAF/CIP1的表达,同时降低cyclinD的表达。结论没药倍半萜化合物抑制前列腺癌细胞的增殖,可能是通过上调p21WAF/CIP1的表达、下调cyclinD蛋白的表达来实现的。Objective To understand the possible mechanisms of two sesquiterpenoids from myrrh on inhibitting proliferation of prostate cancer cells. Methods The growth of prostate cancer cells treated with sesquiterpenoids was examined according to the method of methyl thiazolyl tetrazolium（ MTT）. The cell cycle analysis was performed by flow cytometry. Western blot experiments were employed to detect the expressions of p21^WAF/CIP1 （p21） and cyclin D at protein levels. Transient transfection of pGL3-cyclinD-promoter into the prostate cancer cells was used to analyze the effect of the two compounds on reporter activity of the cyclin D promoter. Results The two sesquiterpenoids had an inhibitory effect on the proliferation of prostate cancer cells, and caused a G0/G1 arrest, induced the expression of p21 and decreased cyclin D. Conclusion Overexpression of p21 might contribute to the two sesquiterpenoid-mediated inhibitory effect on prostate cancer cell growth.

关 键 词：没药 倍半萜 前列腺癌 细胞株 P21 cyclin D 

分 类 号：R963[医药卫生—微生物与生化药学]