石油污染土壤中芘高效降解菌群的筛选及降解特性研究  

Screening and characterization of pyrene-degrading microbial

作　　者：陈晓鹏[1] 易筱筠[1] 陶雪琴[2] 吴仁人[1] 杨琛[1] 党志[1] 

机构地区：[1]华南理工大学环境科学与工程学院,广州510640 [2]仲恺农业技术学院环境科学与工程系,广州510225

出　　处：《环境工程学报》2008年第3期413-417,共5页Chinese Journal of Environmental Engineering

基　　金：广东省科技计划资助项目(2004B20501006);广东省自然科学基金重点项目(05103552)

摘　　要：从长期受石油污染土壤中驯化筛选到能以芘为惟一碳源生长的混合菌群GP3,其主要由假单胞菌株GP3A(Pseudom onassp.)和菌株GP3B(Pandoraea pnom enusa)组成。采用摇瓶振荡培养方法,研究了不同环境条件对混合菌GP3降解芘效能的影响。结果表明,在30℃,150 r/min振荡培养下,混合菌GP3对15 mg/L芘的7 d降解率为90.6%。混合菌GP3降解芘的最适宜温度为35℃,最佳pH值为6.2。加入低浓度葡萄糖(100 mg/L)或菲(10 mg/L)作为共代谢底物,均可提高GP3对芘的降解率。混合菌对芘的降解速率(PDR)与芘的初始浓度呈正相关。研究重金属离子胁迫下GP3对芘的降解时发现,10 mg/L Zn2+的存在对芘降解效能影响较小,Cu2+对芘的降解有抑制作用,Cd2+对混合菌GP3有很强的毒性。A mixed culture, GP3, which could utilize pyrene as the sole carbon and energy source, was enriched from the soil contaminated by polyeyelie aromatic hydrocarbons （PAHs） in the area of Sinopee Guangzhou Petrochemical Complex. In the minimal salt medium（MSM） system, while the initial concentration of pyrene is 15 mg/L, the removal rate of pyrene is approximately 90.6% after 7d. The effects of temperature, initial pH, initial pyrene concentration, heavy metals and the co-metabolism substrates such as glucose and phenanthrene on pyrene degradation by GP3 were investigated. The results showed that the optimal condition was 35℃ and pH 6.2. When the 100 mg/L glucose or 10 mg/L phenanthrene was added into the system with pyrene, they could enhance the degradation of pyrene. A significantly positive correlation was found between initial concentration and pyrene degradation rates. The experimental results also indicated that Zn^2＋ （ 10 mg/L） had no significance on pyrene＇s degradation, but Cu^2＋ could partially inhibit the growth of GP3, and Cd^2＋was toxic to the mixed culture.

关 键 词：多环芳烃 芘 混合菌 生物降解 

分 类 号：X53[环境科学与工程—环境工程]