离子束介导大豆DNA的小麦变异株系苗期酸性蛋白水解酶种类和活性分析  

Analysis of Varieties and Activity of Proteinases of Wheat Irradiated Exogenous DNA from during Seedling Stage

作　　者：张现伟[1] 姬生栋[1] 路素霞[1] 夏云婷[1] 王家传[1] 陈鹏[1] 孟阳[1] 扬刚[1] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007

出　　处：《种子》2008年第2期24-26,共3页Seed

基　　金：河南省自然科学基金资助(0511020600)

摘　　要：采用复性电泳技术对离子束介导大豆DNA小麦变异株系苗期蛋白水解酶进行研究。结果表明:在1叶1心期,两变异株系都检测到了差异酶带,分别为267和214 kD;2叶1心期,变异株系的酶带型与对照没有差异,只是活性的变化;3叶1心期,变异株系间、变异株系与对照的酶带出现很大的差异,酶带表现的活性也不同,个别酶带存在的时期发生了变化。proteinases graph of wheat variants obtained by ion beam implantation with soybean DNA was studied with SDS-G-PAGE technology. The results showed： At the one-leaf stage, bands of 267 and 214 kD were detected in high and low-protein offspring variants respectively; Differential bands wasn＇ t tested and a little change of activity of proteinases was found at the two-leaf stage;At the three-leaf stage, the bands besides the activity were much different between variants and control, and some bands were tested and changed in different stage.

关 键 词：离子束介导 小麦 复性电泳 蛋白水解酶 

分 类 号：S512.1[农业科学—作物学]