体外急性心肌梗死早期诊断方法的研究  

In Vitro Study on the Methods of Early Diagnosis for Acute Myocardial Infarction

作　　者：褚丽萍[1] 刘鉴峰[1] 王彦[1] 胡亚楠[1] 李怀芬[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所天津市分子核医学重点实验室,天津300192

出　　处：《标记免疫分析与临床》2008年第1期32-35,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：从人心肌中提取心肌肌凝蛋白轻链(CMLC),免疫Balb/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞做常规融合,制备单克隆抗体。通过抗人心肌肌凝蛋白轻链单克隆抗体的相加指数、亲和常数测定和抗体夹心实验,筛选到配对较好的两对单抗,在此基础上建立起轻链夹心ELISA方法,为临床急性心肌梗死早期诊断方法研究提供条件。Balb/c mice were immunized with cardiac myosin light chain-1 （CMLC1） purified from human heart. The spleen lymphocytes were taken and fused with SP2/0 mouse myeloma cells to prepare the monoclonal antibody by a routine procedure. By measuring the additive indices, affinity constants and sandwich ELISA test, two pairs of monoclonal antibodies with better quality of combination were selected. The light chain sandwich ELISA method was set up for the diagnosis of acute myocardial infarction.

关 键 词：肌凝蛋白 ELISA 单克隆抗体 轻链 

分 类 号：R542.22[医药卫生—心血管疾病]