A、C群脑膜炎球菌结合疫苗免疫原性研究  

Study on the immunogenicity of group A plus group C meningococcal glycoconjugate vaccine

作　　者：王建华[1] 刘梅影[1] 李冶姗[1] 郭京蓉[1] 张燕斌[1] 陈芸[1] 高华[1] 林云[1] 刘钰[1] 

机构地区：[1]北京生物制品研究所细菌研究室,100024

出　　处：《中华微生物学和免疫学杂志》2008年第2期127-129,共3页Chinese Journal of Microbiology and Immunology

摘　　要：目的研究A、C群脑膜炎球菌结合疫苗的免疫原性，包括剂量、免疫记忆及抗原相容性。方法将不同剂量的A、C群脑膜炎球菌结合疫苗分别免疫小鼠，末次免疫后14d采集血清测定血清抗体滴度，确定最佳免疫剂量后，再分别以单价A群和C群脑膜炎球菌结合疫苗及二价A、C群脑膜炎球菌结合疫苗同时对小鼠分别进行1、2、3针免疫，考察其针次效应和抗原相容性。结果A、C群脑膜炎球菌结合疫苗中的A抗原和C抗原均以1．25μg剂量免疫小鼠时，其免疫原性较好。A、C群脑膜炎球菌结合疫苗能够诱导小鼠产生免疫回忆应答，且在二价结合疫苗中的A群耦联物和C群耦联物配伍后无干扰作用，抗原相容性良好。结论A、C群脑膜炎球菌结合疫苗具有较好的免疫原性和明显的免疫回忆应答，抗原相容性良好。Objective To investigate the immunogenicity of group A plus group C meningococcal glycoconjugate vaccine, namely dosage, immune memory and compatibility. Methods The mice were in jected with group A plus group C meningococcal glycoconjugate vaccine with different dosages. Blood sampies were taken on the 14th day after the last injection for testing the antibodies against polysaccharide A and C. After the optimal immunization dosage had been decided, the mice were inoculated separately with the monovalent group A and the monovalent group C and the bivalent group A plus group C glycoconjugate vaccine with one, two or three injections for observation of the effectiveness of different injections and the compatibilities. Results The dosage of 1.25μg of each polysaccharide of group A and group C in bivalent glycoconjugate vaccine appears to be immunologically optimal to vaccinate the mice. Immunological memory could be induced in mice inoculated with the glycoconjugate vaccine, and the antigenic immunogenicity of the group A component and group C component in the formulation of group A plus group C meningococcal glycoconjugate vaccine was not affected. Conclusion Group A plus group C meningococcal glycoconjugate vaccine have good immunogenicity, immune memory and compatibihty.

关 键 词：A C群脑膜炎球菌结合疫苗 免疫剂量 免疫记忆 抗原相容性 

分 类 号：R686[医药卫生—骨科学]