卡介苗联合Poly I：C接种对新生期小鼠脾脏T细胞的影响  

The co-vaccination effects of BCG and Poly I： C on the development of T cell subsets in neonatal BALB/c mice

作　　者：何云[1] 刘恩梅[2] 杨锡强[1] 刘崇海[1] 李欣[3] 

机构地区：[1]重庆医科大学儿童医院临床免疫室,400014 [2]重庆医科大学儿童医院呼吸科,400014 [3]重庆医科大学儿童医院中心实验室,400014

出　　处：《中华微生物学和免疫学杂志》2008年第2期130-133,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金面上项目（30470760）;国家自然科学基金委青年基金（30300321）;重庆市科委自然科学基金重点项目

摘　　要：目的研究卡介苗（BCG）联合Poly I：C新生期接种对小鼠脾脏T细胞功能亚群发育的影响。方法新生清洁级BALB／c小鼠32只，分4组：对照组、BCG组、Poly I：C组、BCG和Poly I：C联合接种组，新生期接种4周后取脾细胞用流式细胞仪检测CD3^＋CD8^＋IFN-γ^＋、CD3^＋CD8^-IFN-γ^＋、CD3^＋CD8^＋IL-4^＋、CD3^＋CD8^-IL-4^＋、CD4^＋Foxp3^＋T细胞的比例，其分别代表Te1、TH1、Tc2、TH2和调节性T细胞（Treg）亚群。结果BCG组、Poly I：C组、BCG和Poly I：C联合接种组TH1、Tc1细胞比例明显高于对照组（P〈0．05或P〈0．01），3个接种组间比较差异无统计学意义；Tc2、TH2和Treh比例各组间比较差异没有统计学意义；3个接种组TH1／TH2和总IFN-γ／IL-4比值明显高于对照组（P〈0．05或P〈0．01），联合接种组TH1／TH2比值高于BCG组（P〈0．05），Tc1／Tc2比值各组间差异没有统计学意义；CD4^＋Foxp3^＋T细胞比例各组间比较差异没有统计学意义。结论新生期BCG和Poly I：C接种均能促进TH1、Te1细胞亚群的发育，提高TH1／TH2比值，其联合接种在TH1／TH2水平可能具有一定的协同作用，但不能影响CD4^＋Foxp3^＋Treg细胞的比例。Objective To explore the effects of BCG and Poly I： C co-vaccination on the development of spleen T cell subsets of neonatal BALB/c mice. Methods Neonatal BALB/c mice were inoculated with BCG and/or Poly I： C intraperitoneally within 2-3 d after birth. Four weeks later, spleen cells of mice were isolated and the percentage of CD3^＋ CD8^＋ IFN-γ^＋ , CD3^＋ CD8^-IFN-γ^＋ , CD3^＋ CD8^＋ IL-4^＋ , CD3^＋ CD8^-IL-4^＋, CD4^＋ Foxp3^＋ T cells, which represent Tc1, TH1, Tc2, TH2, Treg cells, respectively, were tested by flow cytometry at single cell level, and the ratios of TH1/TH2 and Tc1/Tc2 were calculated. Resuits The percentages of TH1 and Tcl cells of BCG-vaccinated mice, Poly I： C-vaccinated mice and BCG plus Poly I： C-vaccinated mice were significantly higher than that of control mice （ P 〈 0.05 or P 〈 0.01 ）, and there was no difference among the three vaccinated group. The ratios of TH1/TH2 and total IFN-γ/IL-4 of the three vaccinated groups were higher than that of control group, but not the ratio of Tc1/Tc2. The TH1/ TH2 ratio of BCG plus Poly I： C-vaccinated group was higher than that of BCG-vaccinated group （P 〈0.05 ）. The percentages of Treg cells showed no difference among the four groups （ P 〉 0.05 ）. Conclusion BCG and Poly I： C co-vaccination can significantly increase the number of Tc1 and TH 1 cells and TH 1/TH2 ratio in spleen cells. BCG and Poly I： C vaccination may have a synergistic effect on TH1/TH2 ratio of spleen cells in neonatal mice. The percentage of CD4 ^＋ Foxp3^＋ T cells among four groups showed no significant difference.

关 键 词：卡介苗 多聚次黄苷酸-胞苷酸 T细胞亚群 调节性T细胞 

分 类 号：R686[医药卫生—骨科学]