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作 者:何云[1] 刘恩梅[2] 杨锡强[1] 刘崇海[1] 李欣[3]
机构地区:[1]重庆医科大学儿童医院临床免疫室,400014 [2]重庆医科大学儿童医院呼吸科,400014 [3]重庆医科大学儿童医院中心实验室,400014
出 处:《中华微生物学和免疫学杂志》2008年第2期130-133,共4页Chinese Journal of Microbiology and Immunology
基 金:国家自然科学基金面上项目(30470760);国家自然科学基金委青年基金(30300321);重庆市科委自然科学基金重点项目
摘 要:目的研究卡介苗(BCG)联合Poly I:C新生期接种对小鼠脾脏T细胞功能亚群发育的影响。方法新生清洁级BALB/c小鼠32只,分4组:对照组、BCG组、Poly I:C组、BCG和Poly I:C联合接种组,新生期接种4周后取脾细胞用流式细胞仪检测CD3^+CD8^+IFN-γ^+、CD3^+CD8^-IFN-γ^+、CD3^+CD8^+IL-4^+、CD3^+CD8^-IL-4^+、CD4^+Foxp3^+T细胞的比例,其分别代表Te1、TH1、Tc2、TH2和调节性T细胞(Treg)亚群。结果BCG组、Poly I:C组、BCG和Poly I:C联合接种组TH1、Tc1细胞比例明显高于对照组(P〈0.05或P〈0.01),3个接种组间比较差异无统计学意义;Tc2、TH2和Treh比例各组间比较差异没有统计学意义;3个接种组TH1/TH2和总IFN-γ/IL-4比值明显高于对照组(P〈0.05或P〈0.01),联合接种组TH1/TH2比值高于BCG组(P〈0.05),Tc1/Tc2比值各组间差异没有统计学意义;CD4^+Foxp3^+T细胞比例各组间比较差异没有统计学意义。结论新生期BCG和Poly I:C接种均能促进TH1、Te1细胞亚群的发育,提高TH1/TH2比值,其联合接种在TH1/TH2水平可能具有一定的协同作用,但不能影响CD4^+Foxp3^+Treg细胞的比例。Objective To explore the effects of BCG and Poly I: C co-vaccination on the development of spleen T cell subsets of neonatal BALB/c mice. Methods Neonatal BALB/c mice were inoculated with BCG and/or Poly I: C intraperitoneally within 2-3 d after birth. Four weeks later, spleen cells of mice were isolated and the percentage of CD3^+ CD8^+ IFN-γ^+ , CD3^+ CD8^-IFN-γ^+ , CD3^+ CD8^+ IL-4^+ , CD3^+ CD8^-IL-4^+, CD4^+ Foxp3^+ T cells, which represent Tc1, TH1, Tc2, TH2, Treg cells, respectively, were tested by flow cytometry at single cell level, and the ratios of TH1/TH2 and Tc1/Tc2 were calculated. Resuits The percentages of TH1 and Tcl cells of BCG-vaccinated mice, Poly I: C-vaccinated mice and BCG plus Poly I: C-vaccinated mice were significantly higher than that of control mice ( P 〈 0.05 or P 〈 0.01 ), and there was no difference among the three vaccinated group. The ratios of TH1/TH2 and total IFN-γ/IL-4 of the three vaccinated groups were higher than that of control group, but not the ratio of Tc1/Tc2. The TH1/ TH2 ratio of BCG plus Poly I: C-vaccinated group was higher than that of BCG-vaccinated group (P 〈0.05 ). The percentages of Treg cells showed no difference among the four groups ( P 〉 0.05 ). Conclusion BCG and Poly I: C co-vaccination can significantly increase the number of Tc1 and TH 1 cells and TH 1/TH2 ratio in spleen cells. BCG and Poly I: C vaccination may have a synergistic effect on TH1/TH2 ratio of spleen cells in neonatal mice. The percentage of CD4 ^+ Foxp3^+ T cells among four groups showed no significant difference.
关 键 词:卡介苗 多聚次黄苷酸-胞苷酸 T细胞亚群 调节性T细胞
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