螺内酯对急性心肌梗死大鼠左室血流动力学及胶原重构的实验研究  

Effects of spironolactone on left ventricular hemodynamics and collagen remodeling after myocardial infarction in rats.

作　　者：李侠[1] 董士民[1] 刘坤申[2] 

机构地区：[1]河北医科大学第三医院急诊科,石家庄市050051 [2]河北医科大学第一医院心内科

出　　处：《河北医药》2008年第2期136-137,共2页Hebei Medical Journal

基　　金：河北省科技厅计划项目(编号:052761200)

摘　　要：目的研究螺内酯对大鼠心肌梗死(MI)后血流动力学和心肌胶原重构的影响。方法通过结扎冠状动脉左前降支诱导大鼠急性MI模型,心肌梗死后24h将大鼠随机分为:(1)安慰剂组;(2)小剂量螺内酯组;(3)大剂量螺内酯组,另设(4)假手术组,每组8只。于第2周、第6周观察以下指标:(1)平均动脉压、左室舒张末压;(2)非梗死区心肌胶原含量及Ⅰ/Ⅲ型胶原蛋白比值。结果与假手术组相比安慰剂组左室舒张末压均升高(P均<0.01),心肌胶原含量及Ⅰ/Ⅲ型胶原蛋白比值也明显增加(P均<0.01)。与安慰剂组相比螺内酯组左室舒张末压、心肌胶原含量及Ⅰ/Ⅲ型胶原蛋白比值有显著下降(P<0.05或0.01)。与小剂量螺内酯组相比,大剂量螺内酯组左室舒张末压以及各项胶原指标均显著降低(P<0.05或0.01)。结论螺内酯可改善MI后左室舒张功能,抑制非梗死区胶原增生及重建。大剂量螺内酯较小剂量螺内酯效果更明显。Objective To observe the effects of spironolactone on left ventricular function and collagen remodeling after actue myocardial infarction（ MI）. Methods AMI was induced by ligating the left anterior descending coronary artery in rats.24 hours after coronary ligating, the rats were randomly divided into four groups and treated for six weeks. （ 1 ） MI ＋ placebo; （2） MI ＋ small-dose spironolactone（ SSpi ） ; （3） MI ＋ high-dose spironolactone （ LSpi ） ; （4） sham-operation group. The Mean blood press（MAP）, left ventricular end-diastolic press（ LVEDP）, interstitial collagen content and the ratio of type Ⅰ and Ⅲ collagen in non-infarction area were detected at the 2nd week and the 6th week. Results As compared with that in sham-operation group, LVEDP in placebo-treated infracted rats was increasedsingnificantly（ P 〈 0.01 ）, so did the collagen content（ P 〈 0.01 ） and the ratio of type Ⅰ and Ⅲ collagen, as compared with that of sham group at the 2nd week and the 6th week（ P 〈 0.01 ）. In spironolactone treated rats the collagen content, the ratio of type Ⅰ and Ⅲ collagen , MBP and LVEDP were significantly decreased, as compared with those in placebo-treated rats（ P 〈 0.05 or P 〈 0.01 ）, however, L-Spi treated rats were lower in collagen content and the ratio of type Ⅰ and Ⅲ collagen at the 2nd week and the 6th week, and LVEDP at the 6nd week than S-Spi treated rats （ P 〈 0.05）. Conclusion Spironolactone couldimprove the left ventricular function and has beneficial effects on the left ventricular collagen remodeling after myocardialinfarction.

关 键 词：心肌梗死 血流动力学 胶原重构 螺内酯 大鼠 

分 类 号：R542.22[医药卫生—心血管疾病] R540.45[医药卫生—内科学]