不同状态下牙髓组织蛋白质表达的二维电泳分析  

Two-dimensional electrophoresis analysis of the protein expression of dental pulp in different conditions

作　　者：林晨[1] 聂敏[1] 张露[1] 陈智[1] 

机构地区：[1]武汉大学口腔医学院口腔生物医学工程教育部重点实验室,430079

出　　处：《中华口腔医学杂志》2008年第3期154-156,共3页Chinese Journal of Stomatology

基　　金：湖北省青年杰出人才基金（2003ABB019）

摘　　要：目的利用蛋白质组技术探讨牙髓对中龋和热刺激的反应。方法用双向电泳得到牙髓在中龋和热刺激状态下的二维电泳图谱，对差异点进行质谱鉴定。结果经Image Master 2-D Platinum5．0软件分析显示，正常牙髓和中龋牙髓的蛋白表达无显著差异；热损伤牙髓有2个蛋白点缺失，8个蛋白点下调。质谱分析鉴定了7种蛋白质。结论本实验条件下，中龋牙髓的蛋白质表达与正常牙髓无显著差异，热刺激可造成部分蛋白表达的下调。Objective To analyze the different responses of dental pulp to moderate caries and thermal stimulation by proteomics. Methods Two-dimensional electrophoresis （2-DE） was performed to obtain the 2-D gel electrophoresis patterns of dental pulp. Mass spectrometry （MS） was used to analyze several different selected spots in the expression proteins. Results No significant difference in protein expression was found between normal and moderate carious dental. Two protein spots were absent in heatdamaged group and 8 spots showed significantly down-regulated. Seven proteins were identified by MS. Conclusions In the present study, no significant difference in the pulp protein expression was detected between the healthy and moderate carious pulp tissues. However, down-regulation of pulp protein in thermal stimulation was observed.

关 键 词：牙髓 蛋白质组 电泳 凝胶 双向 质谱分析法 

分 类 号：R686[医药卫生—骨科学]