高压氧对缺血再灌注小鼠脑组织中细胞因子TNF-α、IL-6mRNA表达及血脑屏障通透性的影响  

Effects of hyperbaric oxygen on cytokines TNF-α and IL-6 mRNA in the brain tissues and blood brain barrier after cerebral ischemia-reperfusion in mice

作　　者：赵红[1] 李亘松[1] 朱丽娜[1] 张海鹏[2] 陈学新[3] 

机构地区：[1]中国医科大学基础医学院机能实验中心,辽宁省沈阳110001 [2]中国医科大学基础医学院病理生理教研室,辽宁省沈阳110001 [3]中国医科大学基础医学院第一临床医院高压氧科,辽宁省沈阳110001

出　　处：《中华航海医学与高气压医学杂志》2008年第1期9-11,共3页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：辽宁省教育厅基金资助项目（05IA56）

摘　　要：目的探讨高压氧（hyperbaric oxygen，HBO）对缺血再灌注小鼠脑组织中细胞因子TNF-α、IL-6mRNA的表达及血脑屏障通透性的影响。方法复制清醒小鼠脑缺血再灌注模型，并于再灌注期间行0．25MPaHBO治疗5次，在处死动物前1h经尾静脉注射2％伊文思兰（Evans blue，EB），采用RT—PCR及分光光度法分别检测脑组织中细胞因子TNF-α、IL-6mRNA的表达及EB的含量。结果细胞因子TNF-α、IL-6mRNA的表达及EB的含量缺血再灌注组较假手术组高，且2组间差异有统计学意义（P〈0．01）；HBO组与假手术组相比差异无统计学意义（P〉0．05）；HBO＋脑缺血再灌注组与脑缺血再灌注组相比差异有统计学意义（P〈0．01）。结论HBO可从基因水平明显减少脑缺血再灌注细胞因子TNF-α、IL-6mRNA的表达，从而降低血脑屏障通透性，而对正常脑组织中TNF-α、IL-6mRNA表达的影响很小。Objective To investigate effects of hyperbaric oxygen （HBO） on cytokines TNF-α and IL- 6 mRNA in the brain tissues and blood brain barrier （BBB） after cerebral ischemia-reperfusion in mice. Methods Using conscious mice cerebral ischemia-reperfusion models, 0.25 MPa HBO were applied 5 times during the reperfusion period, and 2% Evens blue （EB） was injected into tail veins before an hour when the animals were died. The expressions of TNF-α and IL-6 mRNA as well as the contents of EB were determined by RT-PCR and spectrophotometer. Results The expressions of TNF-α and IL-6 mRNA as well as the contents of EB successively increased in ischemia-reperfusion group compared with the sham surgery group （P 〈 0.01 ） , were similar to those of the sham surgery group （ P 〉 0. 05 ）, were successively decreased compared with ischemia-reperfusion group（ P 〈 0.01 ）. Conclusions HBO can significantly reduce the expressions of TNF-α and IL-6 mRNA and the permeability of BBB, but has little effect on normal cerebral tissues.

关 键 词：高压氧 血脑屏障 缺血再灌注 细胞因子 

分 类 号：R686[医药卫生—骨科学]