低温胁迫下4种苹果砧木叶片多胺的变化  

Changes of polyamines in the leaves of 4 apple rootstocks during low temperature stress

作　　者：赵玲玲[1,2,3] 宋来庆[4] 刘志[1] 谢兴斌[1] 翟衡[1] 郝玉金[1,2] 

机构地区：[1]山东农业大学园艺科学与工程学院 [2]山东农业大学作物生物学国家重点实验室,山东泰安271018 [3]山东烟台市农业科学研究院,山东烟台265500 [4]山东烟台市农业科学研究院

出　　处：《果树学报》2008年第2期151-156,共6页Journal of Fruit Science

基　　金：高等学校全国优秀博士学位论文作者专项资金(200459);新世纪优秀人才支持计划

摘　　要：以山定子(Malus baccata Borkh)、新疆野苹果[M.sieversii(Ledeb)Roem]、烟台沙果[M.prunifoli(Willd)Borkh]和莱芜难咽(M.micromalus Makino)等4种苹果砧木为试材,测定了低温胁迫下不同砧木叶片的多胺含量。结果表明,胁迫6 h时叶片的MDA含量可反映4个砧木的耐寒性,从高到低依次为山定子>新疆野苹果>莱芜难咽>烟台沙果;低温胁迫能明显诱导多胺总量(PAs)、腐胺(Put)、亚精胺(Spd)和精胺(Spm)的合成,耐寒性强的山定子和新疆野苹果PAs、Put和Spd增加显著,而耐寒性较弱的莱芜难咽和烟台沙果多胺和3种胺的变化不大。低温胁迫6 h时,MDA变化量与叶片PAs、Put、Spd变化量以及Put/PAs比值呈极显著或显著负相关,而与(Spd+Spm)/Put、Spd/PAs、Spm/PAs呈显著正相关,与Spm变化量无相关关系,表明当低温胁迫下叶片PAs、Put、Spd增加量较大时,苹果砧木耐低温胁迫的能力比较强,PAs、Put及Spd在胁迫条件下的增加量可作为苹果砧木耐低温胁迫能力高低的鉴定指标。Changes in Maleie dialdehyde （MDA） and polyamines content in the leaves of 4 Malus rootstocks were monitored during low temperature stress. The results showed that MDA content indicated the cold tolerance of 4 Malus rootstocks in 6 hours exposed to low temperature stress. The cold tolerance of 4 rootstocks were ranked as M. baeeata Borkh 〉M. sieversii （Ledeb） Roem 〉M. mieromalus Makino〉M. prunifolia （Willd） Borkh. The contents of putreseine （Put）, spermidine （Spd）, spermine （Spin） and total polyamines were significantly induced by low temperature. Put, Spd and total polyamines rapidly increased in M. baeeata and M. sieversii, while changed a little in M. mieromalus and M. prunifolia. Furthermore, MDA con- tent was negatively correlated with the changes of total polyamine, Put and Spd contents as well as the ratio Put/PAs, while positively with the ratios of Spd/PAs, Spm/PAs, and （Spd＋Spm）/Put. The titers of total polyamines indicated that Put and Spd in leaves exposed to low temperatures changed significantly, and thus could be used as indicators for tolerance of Malus rootstocks, to cold stress.

关 键 词：苹果 砧木 低温胁迫 多胺 MDA 

分 类 号：S661.1[农业科学—果树学]