铁皮石斛的离体培养和快速繁殖  

Culture in vitro and rapid propagation of Dendrobium officinale

作　　者：郑志仁[1] 朱建华 李新国[1] 娄玉霞[1] 黎万奎[3] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]上海市林业总站,上海200072 [3]上海中医药大学中药研究所,上海201203

出　　处：《上海农业学报》2008年第1期19-23,F0003,共6页Acta Agriculturae Shanghai

基　　金：国家"十一五"科技支撑计划项目(2006BAI06A16);上海市科委中药现代化专项(07DZ19723)

摘　　要：以野生铁皮石斛种子为外植体,研究了不同基础培养基、天然附加物和植物生长调节剂对铁皮石斛种子萌发、原球茎增殖与分化、试管苗生根的影响。结果表明,铁皮石斛种子萌发的适宜培养基为MS＋0.5 mg/L NAA＋10%土豆汁;原球茎增殖与分化的适宜培养基为MS＋0.5 mg/L 6-BA＋0.1 mg/L NAA＋10%土豆汁;试管苗生根的适宜培养基为MS＋0.2 mg/L NAA＋10%土豆汁。在此条件下建立的铁皮石斛快速繁殖体系,原球茎在增殖与分化培养基上培养3个月左右,可分化出大量高约1～2 cm的试管苗,转入生根培养基中培养3个月左右后诱导出大量根系,即可进行大田移栽。To establish the protocorm culturing system of Dendrobium officinale,the effects of different basal meida,natural additives and phytohormones on the seed germination,hyperplasia and differentiation of protocorm,and rootage of test-tube seedlings were studied.Results showed that the medium suitable for the seed germination was MS＋0.5 mg/L NAA＋10% potato extract,the medium suitable for protocorm propagation and differentiation was MS＋0.5 mg/L 6-BA＋0.1 mg/L NAA＋10% potato extract,and the medium suitable for rootage of the test-tuble seedlings was MS＋0.2 mg/L NAA＋10% potato extract.

关 键 词：铁皮石斛 组织培养 快速繁殖 

分 类 号：Q943.1[生物学—植物学] S367.7[农业科学—作物栽培与耕作技术]