APRIL在实验性自身免疫性心肌炎大鼠心肌组织中的表达及意义  

Expression of APRIL and Its Significance in the Experimental Autoimmune Myocarditis

作　　者：郑茜[1] 唐其柱[1] 彭晟[1] 沈涤非[1] 初洪刚[2] 郭海鹏[1] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060 [2]武汉大学人民医院超声影像科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2008年第2期193-197,F0003,共6页Medical Journal of Wuhan University

基　　金：武汉市科技计划项目(编号:20056001021-02)

摘　　要：目的:建立Lewis大鼠自身免疫性心肌炎实验模型,探讨增殖诱导配体(APRIL)在实验性自身免疫性心肌炎大鼠心肌组织中的表达及其意义。方法:将提纯的猪心肌肌球蛋白和完全弗氏佐剂等体积混合成乳浊液在1 d8、d免疫具有遗传易感性的Lewis大鼠,而对照组仅用完全弗氏佐剂皮下注射。在免疫后的21 d9、0 d超声心动图测定实验组和对照组大鼠心功能的各项指标(EF%、FS%、LVEDd、LVEDs),取大鼠的心肌组织作病理学检查,采用RT-PCR技术检测APRIL在大鼠心肌组织中的表达情况。结果:HE染色显示初次免疫第21天,大鼠出现心肌炎症,炎症细胞主要分布在血管周围。免疫第90天,大鼠心肌细胞内炎症细胞明显减少,心肌细胞出现纤维化,而对照组大鼠心肌均未见到明显炎性改变。超声心动图显示实验组大鼠心功能状况较对照组明显下降(P<0.05),两实验组大鼠心功能无明显差异(P>0.05)。APRIL在炎症大鼠心肌组织中的表达明显,而在对照组大鼠心肌中APRIL的表达较少(P<0.05)。结论:APRIL在实验性自身免疫性心肌炎大鼠心肌中的表达是上调的,A-PRIL可能参与了自身免疫性心肌炎的发生发展过程。Objective： To establish an animal model of experimental autoimmune myocarditis in Lewis rats and to investigate the expression and its significance of APRIL, a member of TNFSFs, in the experimental autoimmune myocarditis. Methods： Genetically predisposed Lewis rats were immunized with purified myosin plus complete Freund＇s adjuvant （CFA） in equal volume on day 1 and day 8, and the control rats were immunized with CFA only. The indexes of heart function （EF%, FS%, LVEDd and LVEDs） in the model group and control group were detected by echocardiogram on day 21 and day 90 after immunization. Hearts were harvested in both groups after the detection of echocardiogram. Histopathological changes of myocardium were measured by HE staining. The expression of APRIL mRNA was measured by reverse transcription polymerase chain reaction （RT-PCR）. Results： On day 21 after immunization, inflammation was detected in the myocardium of experimental rats and inflammatory cells were mainly distributed around the blood vessels. On day 90, inflammatory cells decreased obviously and the myocardial cell became fibrosis, but no obvious inflammation emerged in the myocardium of control rats. The heart function in model group was worsen than in the control group（P〈0.05）, but there was no significant difference between the two model groups（P〈0.05）. The expression of APRIL mRNA was detected in the myocardium of experimental autoimmune myocarditis much more remarkably than in the normal myocardium（P%0.05）. Conclusion： The expression of APRIL mRNA increases in the myocardium of the experimental autoimmune myocarditis, and maybe play a role in the occurrence and development of autoimmune myocarditis.

关 键 词：增殖诱导配体 心肌炎 自身免疫 细胞免疫 

分 类 号：R542.2[医药卫生—心血管疾病]