猪附红细胞体小鼠感染模型的建立及相关指标检测  

Establishment of Mice Model with Eperythrozoon suis and Hematology Changes

作　　者：余飞[1] 华修国[1] 崔立[1] 袁聪俐[1] 杨志彪[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《上海交通大学学报（农业科学版）》2008年第1期42-45,共4页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家863科研项目(2006AA02Z454);国家自然科学基金项目(30671870)

摘　　要：构建了猪附红细胞体小鼠感染模型,研究了感染后血液常规指标,红细胞免疫功能和脾淋巴细胞转化功能,旨在为附红细胞体病跨种间感染机制研究以及免疫功能变化研究提供借鉴。研究采用分离纯化的猪附红细胞体为感染源,以尾静脉注射方式人工感染SPF小鼠,感染后通过血液压片镜检,PCR检测及透射电镜检测鉴定模型建立成功。同时检测对照组和试验组小鼠的血液常规指标变化,红细胞免疫黏附功能和脾淋巴细胞转化功能变化,发现试验组小鼠红细胞总数减少,血红蛋白含量降低,红细胞比容降低,红细胞免疫黏附功能下降,其他指标没有显著变化。A mouse model of Eperythrozoonosis was established to study the changes of hematology and erythrocyte immunologic function. The purified Eperythrozoon suis was intravenous injected to SPF mice. It was assessed by test under microscope, PCR and transmission electron microscope detection. The hematology biochemical indicator and erythrocyte immune adherence function were evaluated after infection. The mouse model is successfully established. RBC, Hb, HCV are decreased in experimental group. The erythrocyte immune adherence function of mice in experimental group is also decreased.

关 键 词：猪附红细胞体 模型 血液学变化 红细胞免疫 

分 类 号：S828.28[农业科学—畜牧学]