大口鲇和鲇鱼血清蛋白质及同工酶的比较研究  

Comparative Studies on Serum Proteins and Isozymes of Silurus meridionalis Chen and Silurus asotus L.

作　　者：邹桂伟[1] 潘光碧[1] 罗相忠[1] 胡德高[1] 

机构地区：[1]中国水产科学研究院长江水产研究所

出　　处：《遗传》1997年第5期34-36,共3页Hereditas（Beijing)

摘　　要：采用聚丙烯酰胺梯度凝胶垂直板电泳，分析了大口鲇和鲇鱼的血清蛋白质以及心脏、肝脏、眼和肌肉４种组织的ＥＳＴ及ＭＤＨ同工酶。结果表明，大口鲇和鲇鱼的血清蛋白质均能分离出２０条左右的谱带，两者既表现出相同的谱带，又表现出迁移率和含量都不同的带型。两者的ＥＳＴ和ＭＤＨ同工酶在４种组织及血清中均能特异性地表达，存在明显的组织和物种特异性。本文认为肝脏是研究大口鲇和鲇鱼种群生化遗传结构与变异的理想材料，同时还探讨了两种鲇鱼的ＭＤＨ同工酶位点。The serum proteins and isozymes in four tissues (heart, liver, eye and musele) ofS. meridionalis Chen and S. asotus Linnaeus were analyzed by polyacrylamide gradient gel vertical electrophoresis. The isozymes are esteraes (EST) and malate dehytrogenase(MDH). The results showed that electrophoretograme of serum proteins were about 20 protein pattens in two species cattfish, they were either the same protein pattens or the different pattens. Electrophoretogram of isozymes (EST, MDH) in two species catfish indicated tissues and species specificity. Experiment considered that the liver was a good material studied biochemical genetic constitution and variation in species group of S. meridionalis Chen and S. asotus Linnaeus.

关 键 词：鱼纲 大口鲇 鲇鱼 血清蛋白质 同工酶 

分 类 号：Q959.406[生物学—动物学]