眼镜王蛇蛇毒神经毒素CM-11二级结构单元的判定  

Determination of the Secondary Structure of the King Cobra Neurotoxin CM 11

作　　者：庞玉玺[1] 刘卫东[1] 刘爱琢[1] 裴奉奎[1] 

机构地区：[1]中国科学院长春应用化学研究所应用谱学开放实验室

出　　处：《生物化学与生物物理学报》1997年第5期503-509,共7页

基　　金：国家自然科学基金

摘　　要：眼镜王蛇毒液抽提物ＣＭ－１１为含７２个残基的长链神经毒素，对其进行了ＤＱＦ－ＣＯＳＹ，ＴＯＣＳＹ和ＮＯＥＳＹ等一系列２Ｄ－ＮＭＲ谱测定，通过系统地分析各种ＮＯＥ信息、化学位移的分布等数据推测了蛋白质有规律二级结构，最后利用ＭＣＤ主链引导法确定了它的二级结构。其中有三段反平行β折叠股（Ｉ２０～Ｗ２６、Ｒ３７～Ａ４３和Ｖ５３～Ｓ５９）、一段α螺旋构象（Ｗ３０～Ｇ３５）和四个可能的转角（Ｐ７～Ｋ１０，Ｃ１４～Ｇ１７，Ｋ５０～Ｖ５３，Ｄ６１～Ｎ６４），蛇毒神经毒素ＣＭ－１１其他肽片段处在伸展构象。The king cobra neurotoxin CM 11 is a small protein with 72 amino acid residues. After its complete assignments of 1H NMR resonance's were obtained using various 2D NMR technologies, including DQF COSY, clean TOCSY and NOESY, the secondary structure was analysed by studying the various NOEs extracted from the NOESY spectra and the distribution of chemical shifts . The secondary structure was finally determined by MCD as follows:a triple strand antiparallel β sheet with I20-W26, R37-A43 and V53-S59 as its β strands, a short α helix formed by W30-G35 and four turns formed by P7-K10, C14-G17, K50-V53 and D61-N64.

关 键 词：眼镜王蛇 神经毒素CM-11 蛇毒 二级结构 

分 类 号：Q959.620.6[生物学—动物学] R996.3[医药卫生—毒理学]