离体灌流鼠心胞浆游离钙—fura—2测定  

MEASUREMENT OF CYTOSOLIC FREE CALCIUM OF ISOLATED PERFUSION HEARTS WITH FURA-2 METHOD

作　　者：陈英茂[1] 耿建华[1] 詹澄扬[2] 潘敬运[2] 

机构地区：[1]承德医学院 [2]中山医科大学

出　　处：《承德医学院学报》1997年第3期181-184,共4页Journal of Chengde Medical University

摘　　要：本实验建立了一种浮动基点式 fura—2测定离体灌流心脏细胞内游离钙浓度的新模型。本模型中浮动基点式测定法克服了目前 fura—2技术中受细胞内某些物质产生的自身荧光变化干扰的缺点,实现了自动消除细胞自身荧光变化对实验结果的影响,并在此基础上将广泛用于测定游离状态的细胞内游离钙的 fura—2技术推广到了离体灌流心脏,实现了在更接近心肌生理状态的条件下测定其胞浆游离钙。A model of measuring cytosolic free calcium concentration of isolated perfusion hearts with fura-2 fluorescence technology was established on a self-made apparatus.A floating fluorescence base point record method was used in the model.The new recrd methd can autmatically cancel the effect of cell-autofluorescence change on experiment results at any time,therefore solved effectively the interference from the autofluorescence.On the basis Of the new record method,fura-2 fluorescence technology was popularized to isolated perfusion hearts from cell-free state.On the model,myocardium cytosolic free calcium was measured on the condition that myocytes were better cIose to their physiological state.

关 键 词：细胞游离钙 FURA-2 离体灌流心脏 临床检验 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R446.1[医药卫生—基础医学]