黄瓜子叶节离体再生体系的建立  

The Establishment of Regeneration System in Vitro from Cotyledonary Nodes of Cucumis sativus L.

作　　者：崔波[1] 梁芳[1] 程喜梅[1] 袁丽洁[1] 叶永忠[1] 

机构地区：[1]河南农业大学生命科学学院

出　　处：《河南科学》2008年第3期288-290,共3页Henan Science

基　　金：郑州市科技攻关项目(051SGDS17017)

摘　　要：以MS为基本培养基,以黄瓜子叶节为外植体,设置了不同激素浓度,直接诱导出不定芽,建立了黄瓜的离体再生体系.结果表明,最佳芽诱导培养基为MS+BA 1.0 mg/L+IAA 0.1 mg/L+ABA 1.0 mg/L+AgNO3 2.0 mg/L,平均每外植体可诱导出2.9个芽;最佳芽伸长培养基为MS+KT 0.5 mg/L;1/2 MS最适于黄瓜的生根.In this paper, the regeneration system in vitro of Cucumis sativus L. was established by using the cotyledonary nodes as explant. The results show that the medium of MS＋BA 1.0 mg/L＋IAA 0.1 mg/L＋ABA 1.0 mg/L ＋ AgNO3 2.0 mg/L is suitable for adventitious shoots inducing. In this medium averagely 2.9 shoots can be induced each explant. The best shoot elongation medium is MS＋KT 05 mg/L and 1/2 MS is optimum for rooting of regenerative plant.

关 键 词：黄瓜 子叶节 不定芽 再生体系 

分 类 号：Q943.1[生物学—植物学]