藤黄灰链霉菌ECO 00001菌株中寡霉素A和C的分离鉴定及其活性  

Separation and identification of oligomycins A and C from Streptomyces luteogriseus ECO 00001 and their bioactive properties

作　　者：李一青[1] 刘树芳[2] 李铭刚[1] 赵江源[1] 李家瑞[2] 文孟良[1] 

机构地区：[1]云南大学生物资源保护与利用重点实验室,昆明650091 [2]云南省农业科学院农业环境资源研究所,昆明650205

出　　处：《植物保护学报》2008年第1期47-50,共4页Journal of Plant Protection

基　　金：国家“十一五”科技支撑计划(2006BAE01A01-9);国家自然科学基金(20762012);云南省自然科学基金项目(2004C0002Z、2006C0004M、2007C006M)

摘　　要：运用生物活性追踪和色谱分离方法,从藤黄灰链霉菌ECO 00001菌丝体丙酮粗提物中分离得到两个活性化合物。经波谱分析,鉴定为大环内酯类抗生素寡霉素C和A。采用孢子萌发抑制法和菌丝生长抑制法测定寡霉素C和A对5种植物病原真菌的离体抗菌活性。当寡霉素C和A的浓度分别为15μg/mL和5μg/mL时,对百合灰霉病菌、百合炭疽病菌、烟草赤星病菌、稻瘟病菌及水稻恶苗病菌的孢子萌发抑制率均为100%;当浓度分别为100μg/mL和50μg/mL时,对上述5种病原真菌的抑菌圈直径均在10mm以上,化合物II对5个供试植物病原真菌的抑制活性强于化合物I。Two bioactive compounds were isolated from the crude acetone extract of the mycelium of Streptomyces luteogriseus ECO 00001 by bioassay-guided and various chromatographic procedures, they were identified as macrolide antibiotic oligomycins C and A by various spectral analysis. Their antifungal activities against five plant pathogenic fungi were evaluated in vitro by using methods of spore germination inhibition and mycelial growth inhibition. When the concentrations of oligomycins C and A were respectively 15 μg/mL and 5 μg/mL, their spore germination inhibition rates to Botrytis elliptica and Colletotrichum liliacearum of Lilium longiflorum, Alternaria alternate, Pyricularia grisea and Fusarium moniliforme were 100%. Their diameters of inhibition circle against the above five plant pathogenic fungi were all above 10 mm when the concentrations of oligomycins C and A were respectively 100 μg/mL and 50 μg/ mL. The inhibited activities against five plant pathogenic fungi of compound Ⅱ were stronger than those of compound Ⅰ.

关 键 词：藤黄灰链霉菌 寡霉素A 寡霉素C 灰霉病 

分 类 号：S482.2[农业科学—农药学] S436.8[农业科学—植物保护]