柑橘‘不知火’果实上INA细菌的分离鉴定及室内防除  

Isolation and identification of INA bacteria in hybrid ‘Skiranui tangerine’ citrus cultivars and their indoor control

作　　者：汪志辉[1] 廖明安[1] 杨文渊[1] 陈善波[1] 黄蕾[1] 边强吉[1] 

机构地区：[1]四川农业大学林学园艺学院,雅安625014

出　　处：《植物保护学报》2008年第1期51-57,共7页Journal of Plant Protection

基　　金：国家自然科学基金(30671451);教育部"新世纪优秀人才支持计划"(NCET-04-0905)

摘　　要：从目前南方发展的优质杂交柑‘不知火’所面临的霜冻问题出发,分离、纯化四川盆地‘不知火’果实上可能存在的优势冰核活性细菌(ice-nucleation active bacteria,INA)。细菌学鉴定和室内防治试验结果表明:分离出的9株INA细菌4株为Erwinia ananas,5株为Pseudomonas syringaepvs.,其冰核活性均受温度、菌液浓度及pH值的影响。供试的抗霜素1号、石硫合剂、代森锰锌三种化学药剂对所分离的INA细菌室内防除效果均在90%以上,其中以抗霜素1号防除效果最好。同时,在同一生态条件下的‘伏令夏橙’果实上还分离出3株对INA细菌有拮抗作用的拮抗菌。In order to slow down the frost damage of the superior hybrid ＇ Skiranui tangerine＇ （ C. unshiu × C. sinensis）in the southern China, 9 strains of ice nucleation active bacteria （INA） which might cause the frost damage of the fruit were isolated from the ＇ Skiranui tangerine＇ fruit sampled from the Pujiang County in Sichuan Basin. Through the bacteriology identification methods, 4 isolates were identified as Erwinia ananas and 5 isolates were identified as Pseudomonas syringae pvs. The activity of INA bacteria was affected by temperatures, bacteria mixture concentrations and pH values. Three experimental medicaments No. 1 frost-resistant medicament, lime-sulphate medicament and macozeb tested in the indoor controlling experiments, their effect of control were above 90%, and the No. 1 frost-resistant medicament showed the best controlling effects on the INA bacteria. Meanwhile, 3 isolates of antagonistic bacteria of INA bacteria were isolated from ＇Valencia＇ fruit on the same ecological condition.

关 键 词：INA细菌 霜冻 杂交柑 防霜药剂 

分 类 号：S436.66[农业科学—农业昆虫与害虫防治]