不动杆菌整合子基因及其耐药性关系的研究  

Relation of integrons to drug resistance in Acinetobacter spp

作　　者：严冰[1] 盖俊惠[1] 艾辉[1] 高霞[1] 池晓霞[1] 徐小平[1] 

机构地区：[1]广东省深圳市第二人民医院检验科,广东深圳518035

出　　处：《中国卫生检验杂志》2008年第2期306-307,330,共3页Chinese Journal of Health Laboratory Technology

基　　金：广东省科技计划资助项目(2005B36001080);深圳市科技局资助项目

摘　　要：目的：研究整合子系统在不动杆菌中的分布情况,探讨其与该菌耐药性之间的关系。方法：收集深圳市第二人民医院2005～2006年临床标本中分离的不动杆菌122株,采用VITEK-32全自动细菌分析仪测定细菌耐药表型;多重PCR方法检测整合子基因及类型。结果：43.4%（53/122）的不动杆菌检测出1类整合子,未检出2、3类整合子;携带与不携带1类整合子的菌株在耐药性上存在显著性差异（P〈0.05）。整合子阳性菌株更多的表现出多重耐药。结论：不动杆菌中只检出1类整合子,整合子与不动杆菌多重耐药关系密切。Objective：To investigate the distribution of the integrons in Acinetobacter spp.and study the relationship between integrons and drug resistance.Methods：Non-repetitive 122 clinical isolates of Acinetobacter spp.were collected from 2005 to 2006.Susceptibility to 15 different antimicrobial agents was determined by VITEK-32.The integrons were examined by multiple PCR.Results：Class 1 integrons was detected in 43.4%（53/122） of the isolates,no class 2 integrons and class 3 integrons were detected.The integrons positive isolates and integrons negative isolates were significantly different in the resistance to all tested antibiotics except for Cefazolin and Nitrofurantoin.Acinetobacter spp with class 1 integrons had higher incidence of multi-drug resistance than those without.Conclusion：Class 1 integron is only found in Acinetobacter spp isolates.There is close relation of integrons to multi-drug resistance in Acinetobacter spp.

关 键 词：不动杆菌 整合子 耐药性 多重耐药 

分 类 号：R378.99[医药卫生—病原生物学]