雌激素的抗衰老作用及其机制研究  

Anti-senescence effects of estrogen and its mechanism

作　　者：陈国栋[1] 吴赛珠[1] 阮云军[1] 彭慧茹[1] 邢晓雯[1] 孙飞[1] 银孟卓[1] 简政威[1] 

机构地区：[1]南方医科大学附属南方医院心内科,广东广州510515

出　　处：《中国老年学杂志》2008年第5期447-449,共3页Chinese Journal of Gerontology

基　　金：国家973基金项目(2007CB507400)

摘　　要：目的探讨雌激素抗人脐静脉内皮细胞(HUVEC)衰老的作用及其机制。方法用60μmol/L过氧化氢(H2O2)作用HUVEC72h产生诱导型细胞衰老模型,观察17β-雌二醇(E2)对内皮细胞衰老的干预作用。实验分空白组、H2O2刺激组、H2O2+E2组及H2O2+E2+ICI182780组。用β-半乳糖苷酶染色检测细胞的衰老,同时检测细胞线粒体细胞色素C氧化酶的活性,检测细胞内ATP水平及活性氧(ROS)水平,透射电镜观察线粒体结构变化。结果H2O2刺激组β-半乳糖苷酶染色阳性细胞比例增加,细胞衰老明显,同时线粒体细胞色素C氧化酶的活性下降,ATP水平下降,ROS水平增加,线粒体结构受损;而H2O2+E2组能明显减轻上述各种变化;H2O2+E2+ICI182780组能明显阻断E2的各种保护作用。结论E具有抗血管内皮细胞衰老的作用,其作用机制是通过保护线粒体而实现的。Objective To investigate the anti-senescence effects of estrogen on human umbilical vascular endothelial cells （HUVECs） and its mechanism. Methods The induced HUVEC senescence modle was exposed to H2O2 at a concentration of 60 μmoL/L for 72 h to observe the intervention effect of 17β-estradiol （E2） on it. HUVECs were divided into control, H2O2 stimulation, H2O2 ＋ E2 and H2O2 ＋ E2 ＋ ICI 182780 groups. The expression of SA-β-gal in each group was detected through senescence-associated β-gal staining, and complex Ⅳ activity was measured by chromometry, and 2,7-dichlorofluorescein diacetate （DCFH-DA） was used to determine intracellular reactive oxygen species （ROS）, intracellular adenosine triphosphate （ATP） level was quantified with a luciferin-and luciferase-based assay, cellular mitochondrial was observed through transmission electron microscope. Results Compared to control group, H2O2 caused more cellular senescence determined by β-gal staining, caused a decrease in complex Ⅳ activity, intracellular ATP level, elevated intracellular ROS level, and caused damage of mitochondrial. Whereas E2 alleviated the changes above significantly. But ICI 182780 blocked the protective effects of E2. Conclusions E2 has protective effects on mitochondria in HUVECs, which consequently contributes to anti-senescence effects on HUVEC.

关 键 词：雌激素 衰老 内皮细胞 线粒体 细胞色素C氧化酶 

分 类 号：R592[医药卫生—老年医学]