应用分子原位杂交技术检测大鼠烧伤后脏器内皮素 mRNA 表达  

Detection of mRNA expression of endothelin 1 in organs on severe burn rat model with in situ hybridization technique

作　　者：王旭[1] 高建川[1] 罗勤[1] 罗向东[1] 杨宗城[1] 黎鳌[1] 

机构地区：[1]第三军医大学西南医院烧伤研究所

出　　处：《中华外科杂志》1997年第5期313-315,共3页Chinese Journal of Surgery

基　　金：国家自然科学基金

摘　　要：本实验动态观察大鼠烧伤后血浆内皮素（ＥＴ）变化，同时应用分子原位杂交技术检测了大鼠烧伤后心、肺、肝、肾等主要脏器ＥＴ－１ｍＲＮＡ的表达及细胞定位。结果显示，烧伤后血浆内皮素浓度迅速升高，伤后６小时达高峰，２４小时仍维持较高水平。原位杂交显示，烧伤后主要脏器组织ＥＴ－１ｍＲＮＡ表达强度及阳性细胞数迅速增加；其表达峰值的次序为肾、肝、肺、心，且表达阳性细胞种类增加。这可能与内皮素发挥其强大的缩血管作用、调节血流再分布有关，对保证供给休克状态下重要器官的血流灌注有其有益的一面，但ＥＴ－１ｍＲＮＡ的过度表达则可能加重内脏器官的缺血缺氧性损害。After the dynamic observation of postburn endothelin changes in plasma on severe burn rat model,we detected ET 1mRNA expression and cell localization in some organs such as heart, lung, liver and kidney with in situ hybridization technique. We that endothelin concentration in plasma rapidly increased postburn (it reached its climax at 6th hour and maintained fairly high level even at 24th hour postburn). Meanwhile in situ hybridization indicated that ET 1 mRNA expression intensity and number of positive cells rapidly increased postburn.The expression climaxes of which appeared decreasing in turn from kidney,liver,lung to heart and the types of positive cells increased. This may due to the endothelin′s functions of contracting blood vessels and regulating the redistribution of blood flow and be helpful for guaranteeing blood perfusion of important organs under shock state, but the overexpression of ET 1 mRNA may make the ischemic and anoxic damages to organs more severe.

关 键 词：烧伤 内皮素 MRNA 分子原位杂交 

分 类 号：R644[医药卫生—外科学]