应用Hep-2细胞分离泌尿生殖道沙眼衣原体  

Isolation of chlamydia trachomatis from urogenital tract with Hep 2 cells

作　　者：阎玲[1,2,1,2] 冯树异[1,2,1,2] 周劲松[1,2,1,2] 王双元[1,2,1,2] 

机构地区：[1]北京医科大学微生物学系 [2]北京医科大学第三医院皮肤科

出　　处：《中华医学检验杂志》1997年第3期175-177,共3页

基　　金：国家计划生育委员会资助

摘　　要：目的为泌尿生殖道沙眼衣原体感染的诊断提供科学、可靠的依据。方法应用人喉癌上皮细胞（Ｈｅｐ－２细胞）对５０份泌尿生殖道分泌物进行沙眼衣原体分离培养，通过镜下直接观察Ｈｅｐ－２细胞中沙眼衣原体包涵体及活动的原体进行鉴定，同时用相应的细胞培养液进行聚合酶链反应（ＰＣＲ）检测。结果８份标本分离培养为阳性，其对应的ＰＣＲ亦为阳性，检出率为１６％。结论用细胞培养法分离泌尿生殖道沙眼衣原体是诊断其感染的最可靠方法。Objective To provide a scientific reliable basis for the diagnosis of chlamydia trachomatis (CT) infection of the urogenital tract. Methods CT from urogenital specimens of 50 cases were isolated by using Hep 2 cells and identified by observing CT inclusion and “jump” of elementary body under the light microscope. In addition, CT genome was also detected in the cell culture fluid by CT PCR. Results Eight specimens were positive, and the PCR results were identical with cell cultures. The positive rate of CT was 16%. Conclusion CT isolated with Hep 2 cells from urogenital specimens is a specific method for the diagnosis of CT infection of the urogenital tract.

关 键 词：泌尿道感染 沙眼衣原体 HEP-2细胞 细胞培养 

分 类 号：R691.3[医药卫生—泌尿科学]