离子色谱-荧光检测同时测定3种喹诺酮类药物  

Simultaneous determination of 3 quinolone antimicrobial agents by ion chromatography with fluorescence detection

作　　者：宋周虎[1] 胡晓敏[1] 张培敏[2] 朱岩[2] 

机构地区：[1]浙江省海宁卫生学校,浙江海宁314400 [2]浙江大学理学院化学系,杭州310028

出　　处：《中国卫生检验杂志》2008年第1期14-15,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立一种离子色谱-荧光检测方法,用于同时测定3种喹诺酮类药物-诺氟沙星（Norfloxacin NOR）、环丙沙星（Ciprofloxacin CIP）和恩诺沙星（Enoxacin ENO）。方法：选用Dionex OmniPac PAX 500色谱柱,淋洗液为15 mmol/L H2SO4内含有35%（V/V）甲醇,流速1.0 ml/min,荧光检测激发波长和发射波长分别是347和420 nm。结果：在1-100μg/ml浓度范围内,各待测物线性关系良好（r〉0.9995,n=8）,NOR、CIP和ENO的检测限（信噪比S/N=3）分别是50、105、80 ng/ml,NOR和CIP片粉中加样回收率分别是103.5%和100.1%。结论：该方法准确、灵敏、快速,可用于药物制剂和食品中药物残留分析。Objective：To develop a method for simultaneous determination of 3 quinolone antimicrobial agents by ion chromatography with fluorescence detection.Methods：The 3 drugs of norfloxacin（NOR）,ciprofloxacin（CIP） and enoxacin（ENO） were separated by Dionex OmniPac PAX 500 column with an eluent containing 15 mmol/L H2SO4 and 35%（V/V） methanol.The flow rate was 1.0 ml/min and detected with fluorescence with excitation and emission wavelenths of 347 nm and 420 nm.Results：The linear concentration range was 1~100 μg/ml for each agent（r〉0.9995,n=8） and LOD（S/N=3）of NOR,CIP,ENO was 50,105,80 ng/ml,respectively.NOR and CIP also showed good recoveries（103.5% and 100.1% respectively） in tablet powders.Conclusion：The method enables accurate,sensitive,rapid analysis for pharmaceutical preparations and drug residues in foods.

关 键 词：离子色谱 荧光检测 喹诺酮类 诺氟沙星(NOR) 环丙沙星(CIP) 恩诺沙星(ENO) 

分 类 号：O657.75[理学—分析化学]