快速检测O9抗原沙门菌免疫金标层析法的建立及其应用研究  

Gold immunochromatographic assay to detect Salmonellae bearing O9 antigen

作　　者：柳健[1] 焦新安 曹春梅[1] 张敏[1] 丁昌平[1] 包广宇[1] 

机构地区：[1]江苏省扬州市第一人民医院中心实验室,江苏扬州225009 [2]扬州大学,江苏扬州225009

出　　处：《中国卫生检验杂志》2008年第1期42-44,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立一种快速检测O9抗原沙门菌的新方法。方法:利用针对沙门菌O9抗原的两株特异性单克隆抗体,建立一种双抗体夹心检测O9抗原沙门菌的胶体金免疫层析试纸条(GICA)。结果:该试纸条对标准肠炎沙门菌LPS的最小检出量为0.15 ng/条,对肠炎沙门菌菌液的最小检出量为2×105cfu/条。蛋品、鸡肉、猪肉的人工污染试验显示,经增菌后免疫层析试纸条可检出1 cfu的肠炎沙门菌,在大肠杆菌与沙门菌比例为1000:1仍不干扰试纸条的检测。共对市场采集的鸡蛋、鸡肉以及猪肉样品577份进行免疫层析试纸的检测,试纸条与经典细菌分离鉴定法相比:敏感度为90.0%,特异性为98.6%。结论:检测O9抗原沙门菌的GICA法快速、特异、灵敏,在重要沙门菌病检测方面有着潜在、广阔的应用前景。Objective：To develop a quick method for detecting Salmonellae bearing O9 antigen.Methods：A gold immunochromatographic assay（GICA） to detect Salmonellae O9 antigen has been developed by using two different monoclonal antibodies that bound distinct epitopes of Salmonellae O9 antigen.Results：The detection limit for this strip was 0.15 ng Salmonella enteritidis LPS and 2×105 cfu for the strain.The strip could detect 1cfu Salmonella enteritidis after enrichment and E.coli didn′t interfere with the detection even at a ratio of 1000：1 with Salmonella enteritidis.GICA detected 577 samples,Salmonellae O9 antigen was detected by GICA which was in accordance with the classical bacterial separation method,with a sensitivity of 90.0% and a specificity of 98.6%.Conclusion：The GICA is simple,rapid and accurate and can be used in human clinics.

关 键 词：O9抗原沙门菌 胶体金免疫层析法 

分 类 号：R378.22[医药卫生—病原生物学]